Results 151 to 160 of about 17,633 (189)

Tumor Glutaminase Purification

Protein Expression and Purification, 1995
Two alternative purification schemes to obtain the glutaminase from Ehrlich tumor cells in a highly purified form have been developed. One experimental approach is based on conventional and high-performance liquid chromatography fractionation techniques, yielding a 37-fold higher purification than has been previously reported.
J A, Segura, J C, Aledo, S, Gómez-Biedma, I, Núñez de Castro, J, Márquez   +4 more
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Glutaminases

2016
Mammalian glutaminases catalyze the stoichiometric conversion of L-glutamine to L-glutamate and ammonium ions. In brain, glutaminase is considered the prevailing pathway for synthesis of the neurotransmitter pool of glutamate. Besides neurotransmission, the products of glutaminase reaction also fulfill crucial roles in energy and metabolic homeostasis ...
Javier, Márquez, José M, Matés, José A, Campos-Sandoval   +2 more
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Mammalian glutaminase isozymes in brain

Metabolic Brain Disease, 2012
Glutamine/glutamate homeostasis must be exquisitely regulated in mammalian brain and glutaminase (GA, E.C. 3.5.1.2) is one of the main enzymes involved. The products of GA reaction, glutamate and ammonia, are essential metabolites for energy and biosynthetic purposes but they are also hazardous compounds at concentrations beyond their normal ...
Javier, Márquez, Carolina, Cardona, José A, Campos-Sandoval, Ana, Peñalver, Marta, Tosina, José M, Matés, Mercedes, Martín-Rufián   +6 more
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Hormonal control of hepatic glutaminase

Advances in Enzyme Regulation, 1995
(1) Glucagon activates hepatic glutaminase in vivo. Mitochondria from glucagon-injected rats retain an enhanced capacity to catabolize glutamine and this is more sensitive to activation by inorganic phosphate. The glucagon-elicited stimulation of glutaminase is not evident in broken mitochondria.
J T, Brosnan, H S, Ewart, S A, Squires
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Glutaminase Activity and Neocortical Excitability

Nature, 1965
THE work recorded here was carried out in order to ascertain whether glutaminase I activity in the neocortex of the cat plays a part in the glutamate-glutamine system changes induced by the appearance and development of an epileptogenic focus produced by application of mescaline1.
N, Mison-Crighel, C, Pintilie, E, Crighel   +2 more
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Safety evaluation of yeast glutaminase

Food and Chemical Toxicology, 2000
The consumption of soy and soy products (including soy sauce) has been increasing in Western countries due to purported health benefits of soy (cancer protective, estrogenic effects). In addition to providing soy proteins and isoflavones, soy sauce also functions as a flavor enhancer and is able to impart a "umami" taste.
K, Ohshita, Y, Nakajima, J, Yamakoshi, S, Kataoka, M, Kikuchi, M W, Pariza   +5 more
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Glutaminase isozymes in rat kidney

Biochemical and Biophysical Research Communications, 1966
Abstract In 1958 Sayer and Roberts reported on the mode of activation of glutaminase by phosphate using a dog kidney preparation. More recently Kvamme et al. (1965) showed that the activation by phosphate involves a conformational change of the enzyme protein of pig kidney. The authors found that there are two types of glutaminase in rat kidney.
N, Katunuma, I, Tomino, H, Nishino
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MODULATORS OF GLUTAMINASE ACTIVITY

Journal of Neurochemistry, 1972
Abstract— The activating effect of GTP on particulate preparations of glutaminase from rat brain or rat kidney was competitively inhibited by cyclic guanosine 3′,5′‐monophosphate (c‐GMP). Similarly, the effect of ATP was inhibited by cyclic adenosine 3′,5′‐monophosphate (c‐AMP). In the absence of an added activator, the glutaminase activity of brain or
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Endotoxin Stimulates Lymphocyte Glutaminase Expression

Archives of Surgery, 1993
Glutamine is the principal fuel used by lymphocytes. It is hydrolyzed by the glutaminase enzyme, which regulates the rate of intracellular glutamine metabolism. Since lymphocyte glutamine utilization is increased during infection to support cellular proliferation, we hypothesized that endotoxin regulates lymphocyte glutaminase expression at the ...
P, Sarantos, K, Ockert, W W, Souba
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