Results 111 to 120 of about 87,161 (272)

Proteomic Profiling of Primary Hippocampal Neurons Reveals Noncanonical GFAP Expression and Metabolic Adaptations in Glia‐Free Culture

PROTEOMICS, EarlyView.
ABSTRACT Despite their widespread use as a research model, a comprehensive, quantitative proteomic profile of the cultured hippocampal neurons has remained unexplored. Here, we provide the first global proteomic characterization of primary murine hippocampal neurons cultured for 14 days under near‐physiological glucose conditions (2.5 mM).
Dominika Drulis‐Fajdasz, Kinga Gostomska‐Pampuch, Natalia Pudełko‐Malik, Mariusz Fleszar, Agnieszka Gizak, Jacek R. Wiśniewski, Dariusz Rakus   +6 more
wiley   +1 more source

BCKDH: the missing link in apicomplexan mitochondrial metabolism is required for full virulence of Toxoplasma gondii and Plasmodium berghei [PDF]

, 2014
While the apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii are thought to primarily depend on glycolysis for ATP synthesis, recent studies have shown that they can fully catabolize glucose in a canonical TCA cycle.
Barrett, Michael P., Billker, Oliver, Creek, Darren J., Limenitakis, Julien, Macrae, James I., McConville, Malcolm J., Modrzynska, Katarzyna K., Oppenheim, Rebecca D., Pino, Paco, Polonais, Valerie, Seeber, Frank, Soldati-Favre, Dominique   +11 more
core   +1 more source

Thirty years of glyphosate‐resistant crops and weeds: Current situation and future prospects

Pest Management Science, EarlyView.
Since 1996, when the first glyphosate‐resistant crop was commercialized and the first resistant weed was reported, resistance has expanded globally. This review analyzes emergence patterns across weed species, crops, regions, resistance mechanisms, and herbicides.
Ricardo Alcántara‐de la Cruz, Yure Marin Guidi, Mariana Ramirez‐Castillo, Fernando Storniolo Adegas, Caio Antonio Carbonari, Edivaldo Domingues Velini, Rafael De Prado, Stephen O. Duke   +7 more
wiley   +1 more source

An engineered Tetrahymena tRNA(Gln) for in vivo incorporation of unnatural amino acids into proteins by nonsense suppression [PDF]

, 1996
A new tRNA, THG73, has been designed and evaluated as a vehicle for incorporating unnatural amino acids site-specifically into proteins expressed in vivo using the stop codon suppression technique.
Abelson, John N., Dougherty, Dennis A., Du, Fangyong, Kearney, Patrick C., Lester, Henry A., Nowak, Mark W., Saks, Margaret E., Sampson, Jeffrey R.   +7 more
core   +1 more source

Assessment of Oxidative Stress, Vitellogenin, and Human Health Risks Derived From Nile Tilapia Exposed to Metals and Metalloids in Southeastern Brazil

Environmental Toxicology, EarlyView.
ABSTRACT Urban coastal lagoons are highly vulnerable ecosystems increasingly affected by metal contamination, as well as multiple anthropogenic stressors. This study evaluated ecotoxicological and human health risks associated with chronic metal exposure in Nile tilapia (Oreochromis niloticus) from an anthropogenically impacted lagoon in southeastern ...
Julia Araújo Alves, Priscila M. O. Muniz Cunha, Shênia Patrícia Corrêa Novo, Lorena Oliveira Souza Soares, Tatiana Dillenburg Saint‐Pierre, Fábio Veríssimo Correia, André Luís de Sá Salomão, Rachel Ann Hauser‐Davis, Enrico Mendes Saggioro   +8 more
wiley   +1 more source

High‐Altitude Adaptation of Frogs (Case Study: Nanorana parkeri): From Physiological Phenotypes to AltitudeOmics

Integrative Zoology, EarlyView.
High altitudes are challenging for the animals that inhabit these environments. The Xizang plateau frog (Nanorana parkeri) survives in high‐altitude environments through a synergistic strategy of metabolic remodeling and protein expression adjustment to optimize energy efficiency and enhance cellular protection.
Xuejing Zhang, Yonggang Niu, Shengkang Men, Yanghuilin Zhang, Xiaolong Tang, Kenneth B. Storey, Qiang Chen   +6 more
wiley   +1 more source

Clinical‐Grade Human Induced Pluripotent Stem Cell‐Derived Neural Precursor Cells Restore Motor Function and Preserve Striatal Integrity in a Quinolinic Acid‐Lesioned Rat Model of Huntington's Disease

Cell Proliferation, EarlyView.
Clinical‐grade HLA‐homozygous iPSC‐derived neural precursor cells restore motor function, rebuild striatal circuitry and reduce neuroinflammation in QA‐lesioned rats. These findings demonstrate robust neuronal replacement and microenvironment modulation, supporting their potential as a regenerative therapy for Huntington's disease.
Hyeonjoong Jeon, Il‐Shin Lee, Sanghun Lee, Hyo Chang Park, Beomsoo Kim, Hyun Sook Kim, Jihwan Song   +6 more
wiley   +1 more source

Glutamine Synthetase Activity in Rat Epididymis

Experimental Biology and Medicine, 1994
Glutamine synthetase (GS) activity was measured in the caput and cauda regions of rat epididymis. Specific GS activity in the caput was 27-fold higher than that in the cauda. To compare GS activity within the epididymis to that within other tissues, specific and total GS activities were measured in the brain, liver, testes, kidney, and striated muscle.
M D, Kvidera, G B, Carey
openaire   +2 more sources

Energetic stress in combination with impaired fatty acid oxidation induces sequestration of CoA and adaptation of CoA metabolism

The FEBS Journal, EarlyView.
Computational modelling and in vitro liver cell experiments indicate that medium‐chain acyl‐CoA dehydrogenase (MCAD) deficiency causes an accumulation of (especially medium‐chain) acyl‐CoAs at the cost of free CoA (CoASH). A substantial decrease in CoASH impairs flux through many pathways essential for energy homeostasis.
Ligia Akemi Kiyuna, Christoff Odendaal, Madhulika Singh, Albert Gerding, Miriam Langelaar‐Makkinje, Marianne van der Zwaag, Asmara Drachman, Vladimíra Cetkovská, Gaby Liem Foeng Kioen, Anne‐Claire M. F. Martines, Nicolette C. A. Huijkman, Hein Schepers, Bart van de Sluis, Dirk‐Jan Reijngoud, Ody C. M. Sibon, Amy C. Harms, Thomas Hankemeier, Barbara M. Bakker   +17 more
wiley   +1 more source

Comparative evaluation of noncanonical amino acids as site‐specific NMR probes for the complex of E. coli SSB with single‐stranded DNA without isotope labelling

The FEBS Journal, EarlyView.
Genetic encoding of noncanonical amino acids (ncAA) enables their site‐specific installation in proteins. This work compares the nuclear magnetic resonance (NMR) performance of different ncAAs in a large protein−DNA complex (~ 100 kDa) without isotope labelling. The ncAAs deliver readily detectable NMR signals in the complex between E.
Sreelakshmi Mekkattu Tharayil, Haocheng Qianzhu, Elwy H. Abdelkader, Adarshi P. Welegedara, Josemon George, Christoph Nitsche, Thomas Huber, Gottfried Otting   +7 more
wiley   +1 more source