Results 1 to 10 of about 53,702 (285)
Some of the next articles are maybe not open access.
Synthesis and Characterization of the Selenium Analog of Glutathione Disulfide
Analytical Biochemistry, 1993We synthesized the selenium analog of glutathione disulfide by a liquid phase method and named it glutaselenone (i.e., gamma-L-glutamyl-L-selenocysteinylglycine) diselenide. The selenol of selenocysteine was protected by the p-methoxybenzyl group, which was removed by acidolysis with trifluoroacetic acid in the presence of thioanisol. The overall yield
T, Tamura +5 more
openaire +2 more sources
Glutathione mixed disulfides and heterogeneity of chicken hemoglobins
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 19921. Adult chicken hemoglobins Hb A and Hb D interact with glutathione disulfide, GSSG. The major hemoglobin, Hb A, forms at least two new components, termed GHb AI and GHb AII, and Hb D forms at least one, GHb DI. 2. At pH 8.0 and 5 degrees C, glutathione disulfide (GSSG) in a molar excess of 50 x took 6 days to complete the reaction, although at pH 8.6
E, Reischl, A L, Dafré
openaire +2 more sources
Expression of Thermotolerance Following Microinjection of Glutathione Disulfide
Radiation Research, 1988Asynchronous Chinese hamster ovary cells were microinjected with glutathione disulfide (GSSG). Successfully injected cells were scored by coinjecting FITC-dextran with GSSG, followed by fluorescent microscopy. After microinjection, cells were incubated for 2.5 h at 37 degrees C to permit thermotolerance development and then heated at 45 degrees C for ...
C K, Lumpkin +4 more
openaire +2 more sources
Glutathione Disulfide Variability in Normal Human Blood
Analytical Biochemistry, 1994A prevailing opinion is that glutathione disulfide (GSSG) levels in human blood are very low, but many studies have reported variable results. Therefore, our objective was to determine valid processing conditions for GSSG measurement and apply them to normal human blood samples.
B J, Mills, J P, Richie, C A, Lang
openaire +2 more sources
ELECTROPHORESIS, 2014
This report describes modifications to a CZE method developed by Serru et al. (Clinical Chemistry 2001, 47, 1321–1324) for the simultaneous analysis of reduced glutathione (GSH) and glutathione disulfide (GSSG). Lowering the pH of the run buffer (75 mmol/L boric acid, 25 mmol/L bis‐Tris) from pH 8.4 to 7.8 markedly improved GSH peak area ...
James M, Hempe, Jeannine, Ory-Ascani
openaire +2 more sources
This report describes modifications to a CZE method developed by Serru et al. (Clinical Chemistry 2001, 47, 1321–1324) for the simultaneous analysis of reduced glutathione (GSH) and glutathione disulfide (GSSG). Lowering the pH of the run buffer (75 mmol/L boric acid, 25 mmol/L bis‐Tris) from pH 8.4 to 7.8 markedly improved GSH peak area ...
James M, Hempe, Jeannine, Ory-Ascani
openaire +2 more sources
Free Radical Research, 2004
The blood reduced glutathione (GSH)/GSH disulfide (GSSG) ratio is an index of the oxidant/antioxidant balance of the whole body. Nevertheless, data indicating GSH and GSSG physiological levels are still widely divergent, especially those on GSSG, probably due to its low concentration.
Giustarini, D. +4 more
openaire +3 more sources
The blood reduced glutathione (GSH)/GSH disulfide (GSSG) ratio is an index of the oxidant/antioxidant balance of the whole body. Nevertheless, data indicating GSH and GSSG physiological levels are still widely divergent, especially those on GSSG, probably due to its low concentration.
Giustarini, D. +4 more
openaire +3 more sources
Analytical Biochemistry, 1982
Abstract A method for measurement of both glutathione (GSH) and glutathione disulfide (GSSG) in biological samples has been developed by using an isotachophoretic analyzer. The determination of the amount of GSH was carried out by measuring a zone length of GSH in isotachophoresis.
H, Mikasa +3 more
openaire +2 more sources
Abstract A method for measurement of both glutathione (GSH) and glutathione disulfide (GSSG) in biological samples has been developed by using an isotachophoretic analyzer. The determination of the amount of GSH was carried out by measuring a zone length of GSH in isotachophoresis.
H, Mikasa +3 more
openaire +2 more sources
The FASEB Journal, 2002
ABSTRACT Fluorescence microscopy of A549 cells stained with a glutathione (L‐γ‐glutamyl‐Lcysteinylglycine, GSH)‐specific polyclonal antibody displayed uniform staining of the perinuclear cytosol, with the nuclear region apparently lacking GSH staining. This discontinuous staining was confirmed in other cell types and also corroborated
Therese, Söderdahl +7 more
openaire +2 more sources
ABSTRACT Fluorescence microscopy of A549 cells stained with a glutathione (L‐γ‐glutamyl‐Lcysteinylglycine, GSH)‐specific polyclonal antibody displayed uniform staining of the perinuclear cytosol, with the nuclear region apparently lacking GSH staining. This discontinuous staining was confirmed in other cell types and also corroborated
Therese, Söderdahl +7 more
openaire +2 more sources
Determining Glutathione and Glutathione Disulfide Using the Fluorescence Probe o-Phthalaldehyde
Analytical Biochemistry, 2000Because of the importance of glutathione (GSH) and glutathione disulfide (GSSG) in cellular signal transduction, gene regulation, redox regulation, and biochemical homeostasis, accurate determination of cellular glutathione levels is critical. Several procedures have been developed, but many suffer from overestimating GSSG or from cellular substances ...
A P, Senft, T P, Dalton, H G, Shertzer
openaire +2 more sources
Reduced Glutathione and Glutathione Disulfide
2003R W, Browne, D, Armstrong
openaire +2 more sources