Results 181 to 190 of about 103,261 (272)

Engineering plant immune circuit: walking to the bright future with a novel toolbox

open access: yesPlant Biotechnology Journal, Volume 21, Issue 1, Page 17-45, January 2023., 2023
Generating and developing disease‐resistant plant varieties are required to maintain a sustainable food stockpile and the environment. The ability to engineer immune regulatory components through several approaches, such as genetic transformation, CRISPR/Cas‐mediated gene knockdown/knockout, decoy engineering, pathogen effector‐based strategy, RNAi ...
Uyen Thi Vuong   +6 more
wiley   +1 more source

Redesign of Translocon EXP2 Nanopore for Detecting Peptide Fragments

open access: yesSmall Methods, EarlyView.
Nanopore sensing using the EXP2 nanopore from Plasmodium falciparum is enhanced by engineering mutants to reduce electrical noise and improve peptide detection. The EXP2ΔD231 mutant showed over 50% noise reduction and successfully discriminated diverse peptides, highlighting its potential for protein sequencing.
Mitsuki Miyagi   +4 more
wiley   +1 more source

Genome architecture and diverged selection shaping pattern of genomic differentiation in wild barley

open access: yesPlant Biotechnology Journal, Volume 21, Issue 1, Page 46-62, January 2023., 2023
Summary Divergent selection of populations in contrasting environments leads to functional genomic divergence. However, the genomic architecture underlying heterogeneous genomic differentiation remains poorly understood. Here, we de novo assembled two high‐quality wild barley (Hordeum spontaneum K. Koch) genomes and examined genomic differentiation and
Wenying Zhang   +24 more
wiley   +1 more source

Imaging Ligand‐Receptor Interactions at Single‐Protein Resolution with DNA‐PAINT

open access: yesSmall Methods, EarlyView.
A labeling approach for small ligands in DNA‐PAINT super‐resolution microscopy preserving ligand functionality is demonstrated. This method enables the study of ligand‐receptor interactions at single‐protein resolution providing detailed insights into their spatial arrangement and interactions. Here, the interaction of the Epidermal Growth Factor (EGF)
Monique Honsa   +8 more
wiley   +1 more source

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