Results 131 to 140 of about 1,770 (162)

Isolation of glycophorin with deoxycholate

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1979
In a previous communication we reported that human erythrocyte glycophorin prepared by the lithium diiodosalicylate phenol procedure contains approximately 10 mol of lithium diiodosalicylate per mol of glycophorin, and further we showed that this bound lithium diiodosalicylate is difficult to remove by detergents or organic solvents (Romans, A.Y.
J P, Segrest, T M, Wilkinson, L, Sheng
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Monoclonal Antibodies Against Glycophorin A

Hybridoma, 1988
Two mouse IgM monoclonal antibodies, 177.1 and 179.3, are directed against glycophorin A, the major sialoglycoprotein of human erythrocytes. Both antibodies agglutinate blood group M and N erythrocytes equally well, both before and after treatment with neuraminidase or trypsin, but fail to agglutinate erythrocytes treated with papain.
K, Wasniowska, K R, Schroer, M, McGinniss, C, Reichert, D, Zopf   +4 more
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Magnetic resonance study of 13C reductively methylated glycophorin and glycophorin glycopeptides

International Journal of Biological Macromolecules, 1984
Abstract 13C nuclear magnetic resonance (n.m.r.) spectral data for 13C reductively methylated N-terminal tryptic glycopeptides and for 13C reductively methylated N-terminal glyco-octapeptides derived from homozygous glycophorins AM and AN are presented. Their 13C chemical shift data are compared with the previously published 13C n.m.r.
Robert E. Hardy, Batstone-Cunningham Ron L., Marsha E. Daman, Anne M. Holbrooks, Kilian Dill   +4 more
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Glycophorin expression in murine erythroleukaemia cells

Journal of Cell Science, 1989
ABSTRACT We have identified mature and putative precursor forms of glycophorins expressed in a virus-transformed murine erythroleukaemia (MEL) cell line and compared them with their normal erythroblast counterparts. The following differences were found: (1) the two major MEL cell glycophorins (apparent Mr values 29–30 and 43(×103)) have ...
J B, Ulmer, E D, Dolci, G E, Palade
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Glycophorins of human erythroleukemic K562 cells

Archives of Biochemistry and Biophysics, 1987
Glycophorins related to alpha glycophorin, of the human erythrocyte membrane, were isolated from human erythroleukemic K562 cells. The glycophorins were purified using sodium dodecyl sulfate (SDS)/trichloroacetic acid fractionation and Folch and hot phenol extractions. 0.1-0.2 micrograms was obtained/10(8) cells, or approximately a 15% yield.
R E, Silver, A M, Adamany, O O, Blumenfeld   +2 more
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Molecular genetics of glycophorin MNS variants

Transfusion Clinique et Biologique, 1997
The antigens for the MNS blood group system are Glycophorins A and B (GPA,GPB), products of the GPA gene family. The existence of close to 40 variant phenotypes of this blood group system has been documented by serological analyses. Here is summarized the molecular basis for a large number of variants, including all the variants of the Miltenberger ...
O O, Blumenfeld, C H, Huang
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Protein-lipid interactions: Glycophorin and dipalmitoylphosphatidylcholine

Biochemical and Biophysical Research Communications, 1976
Abstract The choline methyl groups of dipalmitoylphosphatidylcholine were enriched with 13 C, and glycophorin extracted from human erythrocytes was included in bilayers of this phospholipid. At temperatures below the transition temperature, the 13 C nuclear magnetic resonance spectra have two components, one sharp and one broad. The sharp signal is
P, Brûlet, H M, McConnell
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Binding of lithium diiodosalicylate to glycophorin

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1978
Glycophorin was purified from human erythrocyte ghosts by the lithium diiodosalicylate -phenol procedure utilizing 125I-labeled lithium diiodosalicylate. The glycophorin preparation was found to contain 8.9 +/- 2.1 mol lithium diiodosalicylate per mol glycophorin.
A Y, Romans, J P, Segrest
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Quantification of glycophorin A and glycophorin B on normal human RBCs by flow cytometry

Transfusion, 2003
BACKGROUND: The quantification of antigens and proteins on RBCs has been achieved by different approaches. Flow cytometry allows the results of the earliest studies to be to reappraised because it offers the possibility of measuring the immunofluorescence intensity of single cells and integrating the individual data of a large number of cells within a ...
Natalia G, de Isla, Bibiana D, Riquelme, Rodolfo J, Rasia, Juana R, Valverde, Jean F, Stoltz   +4 more
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Rat erythrocyte glycophorins can be isolated by the lithium diiodosalicylate method used for other glycophorins

International Journal of Biochemistry, 1992
1. The lithium diiodosalicylate/phenol method, widely employed for the isolation of membrane sialoglycoproteins (glycophorins) from mammalian erythrocytes, was applied for the first time to the purification of homologous glycoproteins from rat erythrocyte membranes. 2. The resulting preparations showed to be composed of four components, fractionated on
A, Herráez, J C, Díez, J, Luque
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