Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus [PDF]
The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb,
Koszinowski, Ulrich H. +4 more
core +1 more source
Generation of Recombinant N-Linked Glycoproteins in E. coli [PDF]
The production of N-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, Escherichia coli. This methods chapter will outline the components and procedures needed to produce N-linked glycoproteins in E.
Strutton B +3 more
openaire +4 more sources
Mutations in the Schmallenberg virus Gc glycoprotein facilitate cellular protein synthesis shutoff and restore pathogenicity of NSs deletion mutants in mice [PDF]
Serial passage of viruses in cell culture has been traditionally used to attenuate virulence and identify determinants of viral pathogenesis. In a previous study, we found that a strain of Schmallenberg virus (SBV) serially passaged in tissue culture ...
Aislynn Taggart +13 more
core +1 more source
In vivo evidence for quasispecies distributions in the bovine respiratory syncytial virus genome [PDF]
We analyzed the genetic evolution of bovine respiratory syncytial virus (BRSV) isolate W2-00131, from its isolation in bovine turbinate (BT) cells to its inoculation in calves.
Bonnet, Marion +5 more
core +1 more source
PSGL-1 Restricts HIV-1 Infectivity by Blocking Virus Particle Attachment to Target Cells
P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric, mucin-like, 120-kDa glycoprotein that binds to P-, E-, and L-selectins. PSGL-1 is primarily expressed on the surface of lymphoid and myeloid cells and is up-regulated during inflammation to mediate ...
Yajing Fu +13 more
doaj +1 more source
Hematopoietic stem cell transplant (HSCT) recipients have a high risk of developing primary varicella-zoster virus (VZV) infection and reactivation. VZV vaccination may prevent infection and reactivation.
Michael Koldehoff +2 more
doaj +1 more source
Structure analysis of biologically important prokaryotic glycopolymers [PDF]
Of the many post-translational modifications organisms can undertake, glycosylation is the most prevalent and the most diverse. The research in this thesis focuses on the structural characterisation of glycosylation in two classes of glycopolymer (
Ventura, Valeria Victoria +1 more
core +1 more source
Tomato spotted wilt virus glycoproteins induce the formation of endoplasmic reticulum- and Golgi-derived pleomorphic membrane structures in plant cells [PDF]
Tomato spotted wilt virus (TSWV) particles are spherical and enveloped, an uncommon feature among plant infecting viruses. Previous studies have shown that virus particle formation involves the enwrapment of ribonucleoproteins with viral glycoprotein ...
Denecke, J. +5 more
core +2 more sources
A drug for the prevention and therapy of tick-borne encephalitis virus is being developed on the basis of the protective chimeric antibody ch14D5a. At the same time, the epitope recognized by this antibody on the surface of glycoprotein E has not been ...
I. K. Baykov +8 more
doaj +1 more source
Anterograde Spread of Herpes Simplex Virus Type 1 Requires Glycoprotein E and Glycoprotein I but Not Us9 [PDF]
ABSTRACT Anterograde neuronal spread (i.e., spread from the neuron cell body toward the axon terminus) is a critical component of the alphaherpesvirus life cycle. Three viral proteins, gE, gI, and Us9, have been implicated in alphaherpesvirus anterograde spread in several animal models and neuron culture systems.
Helen M, McGraw +3 more
openaire +2 more sources

