Semi-synthesis of Glycoproteins from E. coliThrough Native Chemical Ligation
2010Sufficient quantities of homogeneous samples of post-translationally modified proteins are often not readily available from biological sources to facilitate structure-function investigations. Native chemical ligation (NCL) is a convenient method for the production of biologically active proteins from smaller fragments.
Jonathan P, Richardson, Derek, Macmillan
openaire +2 more sources
Anti-β2 glycoprotein I (anti-β2GPI ) antibodies are important contributors to thrombosis, especially in patients with antiphospholipid syndrome (APS). However, the mechanism by which anti-β2GPI antibodies are involved in the pathogenesis of thrombosis is not fully understood.
Wenjing, Zhang +6 more
openaire +2 more sources
Glycoprotein E of pseudorabies virus and homologous proteins in otheralphaherpesvirinae
Archives of Virology, 1994This paper reviews biological properties of glycoprotein E (gE) of pseudorabies virus (Aujeszky's disease virus) and homologous proteins in other alphaherpesvirinae. It focuses on the gene encoding gE, conserved regions in the gE protein and its homologs, the complex of gE and gI, biological functions of gE in vitro and in vivo, the role of gE in ...
openaire +2 more sources
Antibodies to dengue 2 virus E-glycoprotein synthetic peptides identify antigenic conformation
Virology, 1990Eighteen synthetic peptides have now been prepared from dengue (DEN) 2 virus, representing 80% of the extra-membranal domain of the DEN envelope (E)-glycoprotein. These peptides were selected based upon our previous results with Murray Valley encephalitis (MVE) virus, computer analysis of the DEN virus sequence, and the predicted E-glycoprotein ...
J T, Roehrig +4 more
openaire +2 more sources
Radioimmunoassay for the envelope glycoprotein of subgroup E avian leukosis-sarcoma viruses
Virology, 1978Abstract The envelope glycoprotein of subgroup E avian leukosis viruses (gpE) was purified from Rous-associated virus type 0 (RAV-0) and used in double-antibody competition radioimmunoassays (RIA) and radioimmune precipitations (RIP). When embryo extracts from various inbred lines of chickens were tested, the results of assays for chick helper factor
E J, Smith +2 more
openaire +2 more sources
Novel glycoproteins inhibiting the binding of colorectal cancer cells to E-selectin
Glycoconjugate Journal, 1997Novel glycoproteins carrying sialyl-LeA (SLeA) antigens (SL-GP) were isolated from ascites fluid from a patient with colorectal cancer by immunoaffinity chromatography. Their characteristics, including binding capacity to E-selectin, were investigated. SL-GP showed a typical mucin type amino acid composition in which Ser, Thr and Pro together accounted
M, Inoue +4 more
openaire +2 more sources
Nucleotide sequence analysis of varicella-zoster virus glycoprotein E epitope coding regions
Vaccine, 2001Varicella-zoster virus (VZV) glycoprotein E [gE] contains 623 amino acid residues. Fifty percent of the gE gene, codons 39 to 344 that encompasses two epitope coding regions e1 and c1, was sequenced and analyzed for variation among the 30 VZV isolates. A total of eleven isolates showed variance when compared with Dumas VZV strain sequence through base ...
V, Shankar +3 more
openaire +2 more sources
Human colon cancer cells express multiple glycoprotein ligands for E-selectin.
International Journal of Oncology, 2000The interaction between the colon tumor cell surface and the endothelial cell layer is an important component of tumor intravasation, extravasation, and metastasis. Multiple studies suggest that tumor cells may bind to E-selectin expressed on endothelial cells during these processes.
J, Tomlinson +5 more
openaire +2 more sources
Immunological characterisation of the rubella E 1 glycoprotein. Brief report.
Archives of virology, 1986Three epitopes have been identified on rubella virion envelope polypeptide E 1 using monoclonal antibodies. Antibodies to two of the epitopes, E 1EP1 and E 1EP2, show both haemagglutination inhibition and neutralization activities whereas antibodies to the remaining epitope, E 1EP3, show neutralizing activity only.
L, Ho-Terry +3 more
openaire +1 more source
[Purification and identification of recombinant varicella-zoster virus glycoprotein E].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2005To purify and identify recombinant Varicella-Zoster Virus Glycoprotein E.The recombinant plasmid pGEX-VZVgE was induced by isopropyl-beta-D-thiogalactoside (IPTG), the fusion protein was purified with affinity chromatography column; then the purified fusion protein was cleaved by thrombin, and the product's antigenicity was examined by Western blot.The
Fu-min, Li +6 more
openaire +1 more source

