Results 201 to 210 of about 69,901 (250)

Twisting of glycosidic bonds by hydrolases

Carbohydrate Research, 2009
Patterns of scissile bond twisting have been found in crystal structures of glycoside hydrolases (GHs) that are complexed with substrates and inhibitors. To estimate the increased potential energy in the substrates that results from this twisting, we have plotted torsion angles for the scissile bonds on hybrid Quantum Mechanics::Molecular Mechanics ...
Johnson, Glenn, Petersen, Luis, French, Alfred, Reilly, Peter   +3 more
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Formation of glycoside-hydrolases by oral streptococci

Archives of Oral Biology, 1973
Abstract The formation of glycoside-hydrolases by oral streptococci grown in different substrates under different conditions was investigated. A proteose peptone medium was superior to brain-heart infusion broth and Todd-Hewitt broth for production of all enzymes.
C E, Nord, L, Linder, T, Wadström, A A, Lindberg   +3 more
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Strategies for tailoring pH performances of glycoside hydrolases

Critical Reviews in Biotechnology, 2021
Glycoside hydrolases (GHs) exhibit high activity and stability under harsh conditions, such as high temperatures and extreme pHs, given their wide use in industrial biotechnology. However, strategies for improving the acidophilic and alkalophilic adaptations of GHs are poorly summarized due to the complexity of the mechanisms of these adaptations. This
Shu-Fang, Li, Feng, Cheng, Ya-Jun, Wang, Yu-Guo, Zheng   +3 more
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Hierarchical classification of glycoside hydrolases

Biochemistry (Moscow), 2011
This review deals with structural and functional features of glycoside hydrolases, a widespread group of enzymes present in almost all living organisms. Their catalytic domains are grouped into 120 amino acid sequence-based families in the international classification of the carbohydrate-active enzymes (CAZy database).
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Physiological roles of plant glycoside hydrolases

Planta, 2007
The functions of plant glycoside hydrolases and transglycosidases have been studied using different biochemical and molecular genetic approaches. These enzymes are involved in the metabolism of various carbohydrates containing compounds present in the plant tissues. The structural and functional diversity of the carbohydrates implies a vast spectrum of
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Bioprospecting Metagenomics for New Glycoside Hydrolases

2012
To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenomes of natural microbial biomass decay communities may harbor such enzymes.
Jack, Gilbert, Luen-Luen, Li, Safiyh, Taghavi, Sean M, McCorkle, Susannah, Tringe, Daniel, van der Lelie   +5 more
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Diversity of phosphorylases in glycoside hydrolase families

Applied Microbiology and Biotechnology, 2015
Phosphorylases are useful catalysts for the practical preparation of various sugars. The number of known specificities was 13 in 2002 and is now 30. The drastic increase in available genome sequences has facilitated the discovery of novel activities.
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GLYCOSIDE HYDROLASES IN APLYSIA FASCIATA: ANALYSIS AND APPLICATIONS

Biotechnology and Genetic Engineering Reviews, 2008
Glycosylation is considered to be an important reaction for the chemical modification of compounds with useful biological activities. Glycoside hydrolases are biotechnologically attractive enzymes which can be used in synthetic reactions for assembling glycosidic linkages with absolute stereoselectivity at an anomeric centre.
A Trincone, A Tramice, A Giordano, G Andreotti   +3 more
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Glycoside hydrolases: Catalytic base/nucleophile diversity

Biotechnology and Bioengineering, 2010
AbstractRecent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/nucleophile. A variety of mechanisms are used to replace this function, including substrate‐assisted catalysis, a network of several residues, and the use of non‐carboxylate residues or ...
Thu V, Vuong, David B, Wilson
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Purification of some glycoside hydrolases by affinity chromatography

Carbohydrate Research, 1977
Two glycoproteins have been isolated from the cell walls of baker's yeast. One is a glucan-protein complex which has been partially characterised as having a branched carbohydrate structure composed of chains of (1 leads to 3)-linked beta-D-glucosyl residues, some of which are attached by (1 leads to 6)-linkages to the main chain.
M, Edward, R J, Sturgeon
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