Results 201 to 210 of about 191,408 (290)

Simultaneous Visualization of Distinct Posttranslational Modification States of β‐Catenin Using Genetic Code Expansion and Click Chemistry

ChemBioChem, EarlyView.
A method to visualize, within the same cell, three distinct posttranslational forms of β‐catenin: wild‐type, phosphomimetic (T41E), and synthetic T41C‐GlcNAc, using genetic code expansion and fluorescence microscopy is developed. This strategy reveals how modifications at T41 alter β‐catenin localization and stability. A novel strategy based on genetic
Christer Abou Anny, Sébastien Nouaille, Isabelle Huvent, Corentin Spriet, Céline Schulz, Fabrice Bray, Régis Fauré, Tony Lefebvre, Christophe Biot   +8 more
wiley   +1 more source

Integrated Glycosylation Analysis of Immunoglobulin Isotypes Reveals Expanded Humoral Remodeling in Elderly Tuberculosis Infection. [PDF]

Mol Cell Proteomics
Yang YJ, Lee CH, Wang SY, Chuang YK, Chen MX, Shih HC, Tsai IL.   +6 more
europepmc   +1 more source

Unlocking Biomolecule Modification with Light‐Promoted Photocatalyst‐Free Approaches: Current Tools, Challenges, and Perspectives

ChemistryEurope, EarlyView.
This Perspective focuses on photocatalyst‐free, photoinduced modifications of biomolecules, emphasizing transformations mediated by electron donor–acceptor complexes. Mechanistic aspects, current limitations, and future opportunities are discussed, providing insight into the field of bioconjugation.
Milene Hornink, Maria Eduarda C. Thedy, Eugénie Romero, Gary A. Molander   +3 more
wiley   +1 more source

N-Glycosylation of Antibodies: Biological Effects During Infections and Therapeutic Applications. [PDF]

Antibodies (Basel)
Castañeda-Casimiro J, Vallejo-Castillo L, Peregrino ES, Hernández-Solis A, Vázquez-Flores L, Chacón-Salinas R, Wong-Baeza I, Serafín-López J.   +7 more
europepmc   +1 more source

Biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in Pichia pastoris

, 2014
Raquel Fonseca-Maldonado, Alexandre Maller, Éric Bonneil, Pierre Thibault, Carla Botelho-Machado, Richard J. Ward, Maria de Lourdes Teixeira de Moraes Polizeli   +6 more
openalex   +1 more source

Chemical Methods for Peptide and Protein Backbone Cleavage

ChemistryEurope, EarlyView.
Protein cleavage plays essential roles in biology and is powerful for protein manipulation and functionalization as well as in recombinant expression and purification. Here, the historical background and most recent examples of chemical methods for protein and peptide backbone cleavage are discussed (part of the figure was generated in BioRender).
Miguel Angel Alena‐Rodriguez, Christian A. Olsen   +1 more
wiley   +1 more source

Predicting the Impact of Glycosylation on the Structure and Thermostability of <i>Helicobacter pylori</i> Blood Group Binding Adhesin. [PDF]

Biomolecules
Sijmons D, Islas Rios H, Turner BR, Wanicek E, Holien JK, Walduck AK, Ramsland PA.   +6 more
europepmc   +1 more source

Bioorthogonal Chemistry in Biomolecule Quantification: A Review of Reactions and Strategies

Chemistry – A European Journal, EarlyView.
This review summarizes the application of catalyst‐free bioorthogonal reactions: Staudinger ligation, strain promoted azide alkyne cycloaddition, inverse electron‐demand Diels–Alder, and 2‐cyanobenzothiazole cysteine condensation in the semi‐quantitative and quantitative analysis of biomolecules.
Mingze Yang, Shiqi Wang
wiley   +1 more source

Functions of <i>N</i>-Glycosylation-Related Endoplasmic Reticulum Proteins in the Development and Virulence of Plant Pathogens. [PDF]

J Fungi (Basel)
Wang Y, Chen K, Zhang Y, Zhang Z, Tao Z, Ye X.   +5 more
europepmc   +1 more source