Results 211 to 220 of about 406,798 (331)

Gold Nanoparticles Coated With Hydrophobin‐ProteinA Fusion Protein: Development of a Versatile Immunosensing Platform

Biotechnology and Bioengineering, EarlyView.
The biosensor uses a two‐step method involving Ab‐Vmh2/SpA‐AuNPs. Gold nanoparticles are functionalized with Vmh2/SpA, antibodies and then mixed with analytes. HCl induces aggregation in unbound particles enabling detection through spectral analysis based on analyte interaction and nanoparticle aggregation behavior.
Paola Cicatiello, Bartolomeo Della Ventura, Giulia Fichera, Raffaele Velotta, Paola Giardina, Alessandra Piscitelli   +5 more
wiley   +1 more source

Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions

Biotechnology and Bioengineering, EarlyView.
Proteomic analysis of CHO cells under mild hypothermia (31°C) reveals ER stress responses, ubiquitination dynamics, and adaptive mechanisms. Magnetic immuno‐affinity enrichment and mass spectrometry highlight protein regulation differences in non‐producer versus producer cells, offering insights for optimising biopharmaceutical production of IgG1 and ...
David Ryan, Christiana‐Kondylo Sideri, Michael Henry, Selvaprakash Karuppuchamy, Esen Efeoglu, Paula Meleady   +5 more
wiley   +1 more source

Comprehensive and Site-Specific Characterization of Protein N-Glycosylation in AD Samples Reveals Its Potential Roles in Protein Aggregation and Synaptic Dysfunction. [PDF]

Anal Chem
Xu X, Tan H, Yin K, Xu S, Wang Z, Serrano GE, Beach TG, Wang X, Peng J, Wu R.   +9 more
europepmc   +1 more source

Scale‐up of a monoclonal antibody CHO fed‐batch production in stirred tank bioreactors: Effect of hydrodynamic conditions and feeding regimen

Biotechnology Progress, EarlyView.
Abstract Key hydrodynamic‐related parameters such as volumetric power input (P/V), impeller configuration, aeration strategy, and maximum gas sparge rate, as well as an appropriate feeding strategy, must be carefully selected to improve production yields in bioreactor.
Lucas Lemire, Sebastian‐Juan Reyes, Yves Durocher, Robert Voyer, Olivier Henry, Phuong Lan Pham   +5 more
wiley   +1 more source

Decoding Glycosylation in Neurodegenerative Diseases: Mechanistic Insights and Therapeutic Opportunities. [PDF]

FASEB J
Yu H, Chen X, Yang Y, Gu M, Ren K, Wei Z.   +5 more
europepmc   +1 more source

Glycosylation sites of influenza viral glycoproteins: characterization of tryptic glycopeptides from the A/USSR(H1N1) hemagglutinin glycoprotein [PDF]

, 1981
Sukla Basak, David G. Pritchard, Ajit S. Bhown, Richard W. Compans   +3 more
openalex   +1 more source

Dynamic pH profiles drive higher cell‐specific and volumetric productivity

Biotechnology Progress, EarlyView.
Implementation of dyamic pH profiles for increased CHO cell culture productivity. Abstract Mammalian cell cultures in bioreactors rely heavily on critical process parameter control to ensure optimal growth, productivity, and reproducibility to produce recombinant therapeutic proteins. Culture pH has been shown to be a critical parameter that influences
Stephanie R. Klaubert, Dylan G. Chitwood, Danqia Peng, Erin Redman, Ji Young L. Anderson, Nicholas R. Sandoval, Sarah W. Harcum   +6 more
wiley   +1 more source

Mechanism and role of regulated cell death in tumor immunity and immunotherapy

Cancer Communications, EarlyView.
Abstract Cancer immune checkpoint inhibitors (ICIs) have brought breakthroughs, but only about one‐third of cancer patients benefit from ICIs. In recent years, targeting non‐apoptotic regulated cell death (RCD) subtypes, such as ferroptosis, necroptosis, autophagy, cuproptosis, and pyroptosis, has emerged as a novel strategy in cancer therapy due to ...
Jingwen Hu, Yan Li, Bingjie Lian, Yitao Mao, Luqing Zhao   +4 more
wiley   +1 more source

Biosynthesis of mammalian glycoproteins. Glycosylation pathways in the synthesis of the nonreducing terminal sequences.

, 1979
Thomas A. Beyer, James I. Rearick, James C. Paulson, Jean‐Paul Prieels, J. Evan Sadler, Robert L. Hill   +5 more
openalex   +1 more source

Identification of novel neutralizing determinants for protection against HCV

Hepatology, EarlyView., 2022
Identification of novel neutralizing determinants for protection against hepatitis C virus. Abstract Background and Aims HCV evasion of neutralizing antibodies (nAb) results in viral persistence and poses challenges to the development of an urgently needed vaccine.
Garazi P. Alzua, Anne F. Pihl, Anna Offersgaard, Rodrigo Velázquez‐Moctezuma, Carlos R. Duarte Hernandez, Elias H. Augestad, Ulrik Fahnøe, Christian K. Mathiesen, Henrik Krarup, Mansun Law, Jannick Prentoe, Jens Bukh, Judith M. Gottwein   +12 more
wiley   +1 more source