A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for glycosylation of phenolic compounds. [PDF]
Microb Cell Fact, 2020 Background The interest for finding novel β-glucosidases that can improve the yields to produce second-generation (2G) biofuels is still very high. One of the most desired features for these enzymes is glucose tolerance, which enables their optimal ...
Méndez-Líter JA +8 more
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A Fungal Versatile GH10 Endoxylanase and Its Glycosynthase Variant: Synthesis of Xylooligosaccharides and Glycosides of Bioactive Phenolic Compounds. [PDF]
Int J Mol Sci, 2022 The study of endoxylanases as catalysts to valorize hemicellulosic residues and to obtain glycosides with improved properties is a topic of great industrial interest.
Pozo-Rodríguez A +11 more
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Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities. [PDF]
Molecules, 2023 Chitooligosaccharides (COSs) are b-1,4-linked homo-oligosaccharides of N-acetylglucosamine (GlcNAc) or glucosamine (GlcN), and also include hetero-oligosaccharides composed of GlcNAc and GlcN.
Thomas R, Fukamizo T, Suginta W.
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Synthesis and Characterization of a Novel Resveratrol Xylobioside Obtained Using a Mutagenic Variant of a GH10 Endoxylanase. [PDF]
Antioxidants (Basel), 2022 Resveratrol is a natural polyphenol with antioxidant activity and numerous health benefits. However, in vivo application of this compound is still a challenge due to its poor aqueous solubility and rapid metabolism, which leads to an extremely low ...
Pozo-Rodríguez A +11 more
europepmc +2 more sources
Glycosynthase reaction meets the flow: Continuous synthesis of lacto-N-triose II by engineered β-hexosaminidase immobilized on solid support. [PDF]
Biotechnol Bioeng, 2020 The D746E variant of Bifidobacterium bifidum β‐N‐acetyl‐hexosaminidase is a promising glycosynthase (engineered glycosidase deficient in hydrolase activity) for the synthesis of lacto‐N‐triose II (LNT II), a core structural unit of human milk ...
Ruzic L, Bolivar JM, Nidetzky B.
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Glycosylation of Epigallocatechin Gallate by Engineered Glycoside Hydrolases from Talaromyces amestolkiae: Potential Antiproliferative and Neuroprotective Effect of These Molecules. [PDF]
Antioxidants (Basel), 2022 Glycoside hydrolases (GHs) are enzymes that hydrolyze glycosidic bonds, but some of them can also catalyze the synthesis of glycosides by transglycosylation.
Méndez-Líter JA +9 more
europepmc +2 more sources
Transglycosylation Activity of Glycosynthase Mutants of Endo-β-N-Acetylglucosaminidase from Coprinopsis cinerea. [PDF]
PLoS ONE, 2015 Endo-β-N-acetylglucosaminidase (ENGase), which catalyzes hydrolysis of N-linked oligosaccharides, is a useful tool for analyzing oligosaccharide contents of glycoproteins.
Yasunari Eshima +4 more
doaj +2 more sources
Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans. [PDF]
Front Chem, 2020 Asparagine (N)-linked glycosylation is one of the most common co- and post-translational modifications of both intra- and extracellularly distributing proteins, which directly affects their biological functions, such as protein folding, stability and ...
Chao Q +5 more
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Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants from Streptococcus pyogenes Endoglycosidases for Antibody Glycoengineering. [PDF]
Biochemistry, 2018 Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of ...
Tong X, Li T, Li C, Wang LX.
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Synthesis of Glycosides by Glycosynthases. [PDF]
Molecules, 2017 The many advances in glycoscience have more and more brought to light the crucial role of glycosides and glycoconjugates in biological processes. Their major influence on the functionality and stability of peptides, cell recognition, health and immunity ...
Hayes MR, Pietruszka J.
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