Chemo-enzymatic synthesis of long-chain oligosaccharides for studying xylan-modifying enzymes [PDF]
Chemistry – A European Journal, Volume 29, Issue 26, May 8, 2023., 2023 Plant research is hampered in several aspects by a lack of pure oligosaccharide samples that closely represent structural features of cell wall glycans.
Pfrengle, F. +5 more
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Glycosidases in Carbohydrate Synthesis: When Organic Chemistry Falls Short [PDF]
CHIMIA, 2011 Thanks to the stability, good availability, stereoselectivity and broad substrate specificity, oligosaccharide synthesis catalyzed by glycosidases represents an elegant way to complex carbohydrate structures.
Pavla Bojarová, Vladimír Kren
doaj +3 more sources
High-Throughput Recovery and Characterization of Metagenome-Derived Glycoside Hydrolase-Containing Clones as a Resource for Biocatalyst Development. [PDF]
mSystems, 2019 Functional metagenomics is a powerful tool for both the discovery and development of biocatalysts. This study presents the high-throughput functional screening of 22 large-insert fosmid libraries containing over 300,000 clones sourced from natural and ...
Armstrong Z +8 more
europepmc +2 more sources
Glycosyl benzoates as novel substrates for glycosynthases. [PDF]
Org Biomol Chem, 2023 The development of a procedure for the one-pot synthesis of glycosyl benzoates directly from unprotected sugars in aqueous media using 2-chloro-1,3-dimethylimidazolium chloride (DMC), thiobenzoic acid, and triethylamine is reported. These glycosyl donors
de Lorenzo S +3 more
europepmc +5 more sources
Chemoenzymatically Synthesized O-Acetylated GD3 Gangliosides to Examine Viral Receptor Specificities in a Cellular Context. [PDF]
Angew Chem Int Ed Engl7‐O‐, 9‐O‐, and 7,9‐di‐O‐acetylated GD3 gangliosides can be prepared by chemical assembly of a tetrasaccharide as α‐glycosyl fluoride that is coupled to sphingosine using a glycosynthase, followed by O‐acetyl editing by coronaviral hemagglutinin‐esterases (HEs).
Zhang Z +5 more
europepmc +2 more sources
Computer Simulation to Rationalize "Rational" Engineering of Glycoside Hydrolases and Glycosyltransferases. [PDF]
J Phys Chem B, 2022 Glycoside hydrolases and glycosyltransferases are the main classes of enzymes that synthesize and degrade carbohydrates, molecules essential to life that are a challenge for classical chemistry.
Coines J, Cuxart I, Teze D, Rovira C.
europepmc +3 more sources
Size-controlled synthesis of β(1→4)-GlcNAc oligosaccharides using an endo-glycosynthase.
Chemistry, 2021 Chitin and peptidoglycan fragments are well recognized as pathogen associated molecular patterns (PAMPs). Long-chain oligosaccharides of β(1→4)-linked N -acetyl-D-glucosamine (GlcNAc) units indeed activate plants and mammals innate immune system. However,
A. Rousseau +3 more
semanticscholar +1 more source
Israel Journal of Chemistry, Volume 63, Issue 10-11, October 2023., 2023 Abstract Antibody drug conjugates (ADC) are an emerging class of pharmaceuticals consisting of cytotoxic agents covalently attached to an antibody designed to target a specific cancer cell surface molecule followed by internalization and intracellular release of payload to exhibit its anticancer activity.
Vidya S Shivatare +5 more
wiley +1 more source
Microbial Biotechnology, Volume 16, Issue 10, Page 1971-1984, October 2023., 2023 A monoglycosyl nosiheptide, which exhibit improved solubility and stability compared to nosiheptide, was created by enzymatic glycosylation. By enzyme engineering coupled with a new colorimetric method, a chimeric enzyme with approximately 70 times higher activity was constructed. These findings suggest the potential for structural modifications at the
Ling Zhao +8 more
wiley +1 more source
Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities. [PDF]
PLoS ONE, 2015 Many therapeutic antibodies have been developed, and IgG antibodies have been extensively generated in various cell expression systems. IgG antibodies contain N-glycans at the constant region of the heavy chain (Fc domain), and their N-glycosylation ...
Masaki Kurogochi +14 more
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