Results 31 to 40 of about 1,170 (199)
Challenges and Opportunities for the Large-Scale Chemoenzymatic Glycoengineering of Therapeutic N-Glycosylated Monoclonal Antibodies [PDF]
, 2022 Variability in the glycosylation profile of therapeutic monoclonal antibodies (mAbs), due to recombinant production technologies, leads to inconsistencies in effector functions and pharmacokinetic properties, both batch-to-batch and within single batches.
Falcioni, Francesco, Ivanova, Adelina
core +1 more source
Glycosynthase-catalysed formation of modified polysaccharide microstructures [PDF]
Biochemical Journal, 2004 Glycosynthases are mutant glycosidases, genetically engineered to catalyse the synthesis of oligosaccharides. A study by Faijes et al. in this issue of the Biochemical Journal has expanded the ability of glycosynthases to catalyse the polymerization of carbohydrates to form unnatural oligosaccharide products that can attain unique crystalline forms ...
Andrew G, Watts, Stephen G, Withers
openaire +2 more sources
Glycoside hydrolases from (hyper)thermophilic archaea: structure, function, and applications [PDF]
, 2023 : (Hyper)thermophilic archaeal glycosidases are enzymes that catalyze the hydrolysis of glycosidic bonds to break down complex sugars and polysaccharides at high temperatures.
Cobucci-Ponzano B. +4 more
core +1 more source
Engineering the functional fitness of transglycosidases and glycosynthases by directed evolution [PDF]
, 2013 The artificial implementation of the Darwinian theory of evolution to create new variants of functional proteins, a process referred to as directed evolution, has acquired many applications in biochemical engineering.
Bulimo, WD, Mulaa, FJ, Osanjo, GO
core +2 more sources
Organic and biomolecular chemistry, 2019 Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase.
Katharina Schmölzer +3 more
semanticscholar +1 more source
Sugars : burden or biomaterials of the future? [PDF]
, 2010 During the past few years, the field of tissue engineering (TE) has been shifting from replacement to regenerative strategies. Following this tendency, the requirements for biomaterials to be used in TE have been also changing.
Pashkuleva, I., Reis, R. L.
core +1 more source
Journal of Biological Chemistry, 2016 Chemoenzymatic synthesis is emerging as a promising approach to the synthesis of homogeneous glycopeptides and glycoproteins highly demanded for functional glycomics studies, but its generality relies on the availability of a range of enzymes with high ...
John P. Giddens +3 more
semanticscholar +1 more source
Reagent Engineering for Group Transfer Biocatalysis
Angewandte Chemie, Volume 136, Issue 7, February 12, 2024.Biocatalytic group transfer reactions can efficiently generate highly complex molecules under mild and environmentally friendly conditions. This Minireview highlights inspiring examples of the development of non‐natural donor reagents to power such enzymatic transformations, while discussing the design aspects behind the choice of reagent.
John H. Reed, Florian P. Seebeck
wiley +2 more sources
Organic and biomolecular chemistry, 2019 Correction for 'Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum' by Katharina Schmölzer et al., Org. Biomol. Chem., 2019, DOI: 10.1039/c9ob00424f.
Katharina Schmölzer +3 more
semanticscholar +1 more source
Generation of a Mutant Mucor hiemalis Endoglycosidase That Acts on Core-fucosylated N-Glycans [PDF]
, 2016 Endo-β-N-acetylglucosaminidase M (Endo-M), an endoglycosidase from the fungus Mucor hiemalis, is a useful tool for chemoenzymatic synthesis of glycoconjugates, including glycoprotein-based therapeutics having a precisely defined glycoform, by virtue of ...
Katayama, Takane +6 more
core +1 more source