Results 41 to 50 of about 1,170 (199)
Using Carbohydrate Interaction Assays to Reveal Novel Binding Sites in Carbohydrate Active Enzymes [PDF]
, 2016 Carbohydrate active enzymes often contain auxiliary binding sites located either on independent domains termed carbohydrate binding modules (CBMs) or as so-called surface binding sites (SBSs) on the catalytic module at a certain distance from the active ...
Abou Hachem, Maher +6 more
core +3 more sources
Biocatalytic β-glucosylation/β-galactosylation of Rebaudioside C by glycosynthases
Food Materials ResearchExtracts of Stevia rebaudiana Bertoni plant leaves contain a mixture of diterpene glycosides which possess high sweetness and are used as non-caloric sweeteners in foods and beverages.
Ye-Yang Yu +7 more
doaj +1 more source
Enzymatic conversion of β-mannans: Analysing, evaluating and modifying transglycosylation properties of glycoside hydrolases [PDF]
, 2022 Retaining glycoside hydrolases are enzymes that catalyse the breakdown down of glycans through hydrolysis. Due to the double-replacement mechanism of the retaining glycoside hydrolases (GHs), which form an intermediate with part of the glycan covalently ...
Wiemann, Mathias
core
The First Glycosynthase Derived from an Inverting Glycoside Hydrolase [PDF]
Journal of Biological Chemistry, 2006 Reducing end xylose-releasing exooligoxylanase (Rex, EC 3.2.1.156) is an inverting GH that hydrolyzes xylooligosaccharides (> or = X3) to release X1 at their reducing end. The wild-type enzyme exhibited the Hehre resynthesis hydrolysis mechanism, in which alpha-X2F was hydrolyzed to X2 and HF in the presence of X1 as an acceptor molecule.
Yuji, Honda, Motomitsu, Kitaoka
openaire +2 more sources
, 2019 Background: The interest for finding novel b-glucosidases that can improve the yields for the production of second-generation (2G) biofuels is still very high.
J. A. Méndez-Líter +8 more
semanticscholar +1 more source
ChemSusChem, Volume 18, Issue 20, October 23, 2025.Developing a process to produce glycosylated natural products involves many decisions, and given the current climate crisis, the environmental impact of the various processes must be a major consideration. This review provides an overview of the various processes and a framework for which metrics should be quantified to select processes that are both ...
Felipe Mejia‐Otalvaro +3 more
wiley +1 more source
Expanding the Repertoire of Glycosynthases
Chemistry & Biology, 2009 As reported in this issue, Moracci and coworkers have now expanded the glycosynthase concept to two retaining alpha-L-fucosidases. The newly generated alpha-fucosynthases can use beta-L-fucosyl azide as donors for transglycosylation, enabling the synthesis of fucose-containing oligosaccharides.
openaire +2 more sources
An Update: Enzymatic Synthesis for Industrial Applications
Angewandte Chemie International Edition, Volume 64, Issue 27, July 1, 2025.Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Thomas Bayer +4 more
wiley +1 more source
α-Glucosidase Mutant Catalyzes “α-Glycosynthase”-type Reaction [PDF]
Bioscience, Biotechnology, and Biochemistry, 2002 Replacement of the catalytic nucleophile Asp481 by glycine in Schizosaccharomyces pombe alpha-glucosidase eliminated the hydrolytic activity. The mutant enzyme (D481G) was found to catalyze the formation of an alpha-glucosidic linkage from beta-glucosyl fluoride and 4-nitrophenyl (PNP) alpha-glucoside to produce two kinds of PNP alpha-diglucosides ...
Masayuki OKUYAMA +4 more
openaire +2 more sources
Mass Spectrometry Reviews, Volume 44, Issue 3, Page 213-453, May/June 2025.Abstract The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022.
David J. Harvey
wiley +1 more source