Results 121 to 130 of about 117,081 (314)

Developmentally Guided Differentiation of Ventral Midbrain Dopaminergic Cells Validated in Proteopathy‐Based Parkinson's Disease Models

Advanced Science, EarlyView.
A developmentally guided protocol reproducibly specifies EN1‐positive ventral midbrain dopaminergic (vmDA) progenitors from human pluripotent stem cells and yields functional vmDA neurons. Optogenetic induction of α‐synuclein aggregation elicits selective degeneration of TH‐positive neurons in 2D cultures and 3D neurospheroids, while transplanted ...
Heechang Moon, Heejeong Kim, Elliot H. Lee, Hanseul Kim, Ji Hun Kim, Huisu Jeong, Jiwon Cheon, Ga Ram Jeong, Byoung Dae Lee, Jae‐Won Cho, Soo‐Jin Oh, Yunjong Lee, Yohan Oh   +12 more
wiley   +1 more source

Acoustically Activatable Drug‐Loaded Nanodroplets for Mechanochemical Therapy in Solid Tumors

Advanced Science, EarlyView.
Microfluidically fabricated drug‐loaded nanodroplets are sequentially activated by dual‐frequency ultrasound through acoustic droplet vaporization followed by bubble collapse, enabling spatially controlled drug release and mechanical tumor fractionation, resulting in synergistic mechanochemotherapy and prolonged survival.
Tiran Bercovici, Mike Bismuth, Meir Goldsmith, Dan Peer, Tali Ilovitsh   +4 more
wiley   +1 more source

Sarbecoviruses Suppress Innate Immunity by Recruiting PPM1A to Modulate STAT2 and ORF9b Phosphorylation

Advanced Science, EarlyView.
Sarbecovirus ORF9b recruits host phosphatase PPM1A to suppress innate immunity through dual mechanisms. PPM1A directly dephosphorylates ORF9b to sustain its activity and indirectly downregulates STAT2 phosphorylation to impair interferon signaling. Pharmacological inhibition of PPM1A restores antiviral responses and limits sarbecovirus replication ...
Lixiang Xie, Ziye Huang, Zhiyuan Zhang, Yiqiang Zhu, Xiaoqing Liu, Lei Wang, Tongyu Bi, Taizhen Liang, Jintao Lai, Meilin Hu, Guochang Qiu, Shiqi Xiao, Sen Liu, Yaoming Liu, Haiyue Rao, Tao Chen, Haojie Peng, Bin Zhang, Jia Tang, Qianying Li, Yaxin Li, Yuxin Hou, Weibo Yang, Hewei Jiang, Xiancai Ma   +24 more
wiley   +1 more source

Topical issues of training of GMP inspectors [PDF]

, 2016
Рассмотрены и проанализированы основные требования к системе качества GMP-инспектората в части надлежащей подготовки инспекторов, обобщены международные рекомендации в этой сфере. Показано, что подготовку инспекторов следует организовывать и проводить на
Шестаков, В. Н., Підпружников, Ю. В., Подпружников, Ю. В., Shestakov, V. N., Pidpruzhnykov, Yu. V.   +4 more
core  

Oxygen Partial Pressure as a Kinetic Descriptor for Single‐Crystal Morphology in Mid‐Nickel Layered Cathodes

Advanced Science, EarlyView.
Scalable synthesis of single‐crystal mid‐nickel cathodes demands precise kinetic control over competing densification and grain growth. Oxygen partial pressure emerges as a quantitative descriptor of effective grain‐boundary mobility, acting through changes in the apparent sintering activation barrier.
Hyeonho Park, Sushil Kumar, Sangbeom Kim, Ho Choi, Youngmin Jeon, Taeju Bak, Jisook Hong, M. P. Gururajan, Youngjin Kim, Kyoungdoc Kim   +9 more
wiley   +1 more source

Kiteplatin: Differential binding between GSH and GMP

, 2016
Glutathione (GSH) plays an important role in the development of resistance to platinum-based chemotherapy, since it can prevent drug binding to DNA and resulting apoptosis of tumor cells.
Hoeschele, James D., NATILE, Giovanni, Petruzzella, Emanuele, MARGIOTTA, NICOLA, CURCI, ALESSANDRA   +4 more
core   +1 more source

Decoding Biomolecular Phase Separation: From Physiological Roles to Pathological Mechanisms and Therapeutic Biomaterials Design

Advanced Science, EarlyView.
This review systematically elaborates on the mechanisms underlying physiological regulation and pathological dysregulation of biomolecular phase separation. It also summarizes advances in phase‑separation‑targeted therapeutic strategies and the design of functional biomaterials inspired by this process.
Jiawei Zhu, Jingbo Dong, Qiufang Gong, Xuejiao Song, Zhifa Shen, Chao Liang   +5 more
wiley   +1 more source

A ROS‐Responsive and Mucoadhesive Thermosensitive Hydrogel Encapsulated with Mizoribine for Synergistic Dry Eye Therapy

Advanced Science, EarlyView.
The ROS‐responsive in situ thermosensitive MZR@SPPP hydrogel system enables dual‐stage drug loading and sustained ocular delivery. Upon administration, it gelates at ocular surface temperature, enhancing ocular adhesion. By scavenging free radicals, restoring NRF2‐mediated antioxidant defense and mitochondrial homeostasis, and suppressing inflammatory ...
Danyi Qin, Jiani Li, Panqin Ma, Shinan Wu, Wenying Guan, Yimin Huang, Ke Feng, Mingyan Wei, Zixuan Yang, Shujia Guo, Caihong Huang, Yun‐Long Wu, Yi Han, Zuguo Liu   +13 more
wiley   +1 more source

Preparation and functional studies of FimXEAL-c-di-GMP and FimXEAL-c-di-GMP-PilZ complexes from Xanthomonas campestris

, 2014
c-di-GMP是細菌中一個重要的訊息傳遞分子，參與調控細胞中許多重要的生物功能，包括毒性因子的表達、生物膜的合成以及細胞的運動…等，而其濃度則由GGDEF 和EAL domain負責調節；GGDEF domain具有 Diguanylate Cyclases (DGC) 活性以合成c-di-GMP，而EAL domain 則具有 Phosphodiesterases (PDE)活性以分解c-di-GMP。在先前的文獻中，已得知當細菌中c-di-GMP濃度高時，非典型第二型PilZ ...
廖怡婷, Liao, Yi-Ting
core   +1 more source

Cellular Mechanisms Enabling Mitochondria Transfer and Transplantation

Advanced Science, EarlyView.
Mitochondrial transfer and transplantation (mitoTT) is examined as a multistep process governed by distinct transfer routes and post‐transfer checkpoints. Mechanisms controlling mitochondrial uptake, network integration, cristae remodeling, mtDNA maintenance, immune sensing, and quality control are highlighted, revealing key biological and ...
F. Seyma Gokdemir, Yagmur Ozendi, Gokhan Burcin Kubat, Keshav K. Singh   +3 more
wiley   +1 more source