Green Fluorescent Protein: discover, structure, purification, applications, and future prospects
Journal of Scientific Research in Science, 2023 Green fluorescent protein (GFP) Firstly discovered from the jellyfish Aequorea victoriaand it was widely used in biological science as a universal fluorescent tag.
Hend A. Anter +2 more
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Fluorescence polarization of green fluorescence protein [PDF]
Proceedings of the National Academy of Sciences, 2002 We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué +4 more
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R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. [PDF]
PLoS ONE, 2012 Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level.
You-Tzung Chen +21 more
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Engineering an efficient and bright split Corynactis californica green fluorescent protein
Scientific Reports, 2021 Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even ...
Hau B. Nguyen +2 more
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The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy [PDF]
, 2021 To resolve the signaling mechanisms that mediate the starvation-induced processes of Dictyostelium sporulation and encystation, we performed insertional mutagenesis on cells harboring an mRFP-tagged spore gene.
Schaap, Pauline, Yamada, Yoko
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Fluorescent-Antibody Targeting of Insulin-Like Growth Factor-1 Receptor Visualizes Metastatic Human Colon Cancer in Orthotopic Mouse Models. [PDF]
, 2016 Fluorescent-antibody targeting of metastatic cancer has been demonstrated by our laboratory to enable tumor visualization and effective fluorescence-guided surgery.
Bouvet, Michael +5 more
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Green Fluorescent Protein-Mediated Biomineralization of Silver Microparticles
Orbital: The Electronic Journal of Chemistry, 2021 Biomineralization is a bio-inspired technique of creating inorganic nanostructures using peptides or proteins. An important consideration in selecting a biomineralization agent is the overall shape or geometry of the protein since this can influence the
Ma. Monica M. Cabiles +2 more
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Using photoactivatable fluorescent protein Dendra2 to track protein movement
BioTechniques, 2007 Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov +2 more
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Evolution of a fluorinated green fluorescent protein [PDF]
, 2007 The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine.
A. J. Link +23 more
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A general strategy to red-shift green fluorescent protein based biosensors
bioRxiv, 2020 Compared with green fluorescent protein-based biosensors, red fluorescent protein (RFP)-based biosensors are inherently advantageous because of reduced phototoxicity, decreased autofluorescence and enhanced tissue penetration. However, existing RFP-based
S. Zhang, Hui-wang Ai
semanticscholar +1 more source