Results 61 to 70 of about 1,124,962 (295)
Photoacid behaviour in a fluorinated green fluorescent protein chromophore:Ultrafast formation of anion and zwitterion states [PDF]
, 2016 The photophysics of the chromophore of the green fluorescent protein in Aequorea victoria (avGFP) are dominated by an excited state proton transfer reaction.
Baranov +32 more
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Fluorescent proteins as tools to aid protein production
Microbial Cell Factories, 2005 Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
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Green fluorescent protein: A perspective [PDF]
Protein Science, 2011 AbstractA brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ...
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Subcellular localization of the five members of the human steroid 5α-reductase family [PDF]
, 2017 In humans the steroid 5a-reductase (SRD5A) family comprises five integral membrane enzymes that carry out reduction of a double bond in lipidic substrates: D4-3-keto steroids, polyprenol and trans-enoyl CoA.
Asteriti, ITALIA ANNA +10 more
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Getting to grips with ammonium
eLife, 2013 A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.
Yi Wang, Jonathan A Javitch
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Green Fluorescent Protein Glows Gold [PDF]
Cell, 2008 The awarding of this year's Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discovery and development of green fluorescent protein earns this humble jellyfish protein a place of honor in the biology research hall of fame.
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Green‐fluorescent protein mutants with altered fluorescence excitation spectra [PDF]
FEBS Letters, 1995 Using random mutagenesis and visual selection of fluorescent clones, we have isolated a T2031 and a E222G mutant of the Aequorea green‐fluorescent protein. Each mutant has one of the two fluorescence excitation bands of the wild type deleted and retains the other without a wavelength shift.
Ehrig, Torsten +2 more
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, 2001 To track malaria parasites for biological studies within the mosquito and mammalian hosts, we constructed a stably transformed clonal line of Plasmodium berghei, PbFluspo, in which sporogonic and pre-erythrocytic liver-stage parasites are autonomously ...
Hafalla, JC +5 more
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Variants of Green Fluorescent Protein GFPxm [PDF]
Marine Biotechnology, 2006 As research progresses, fluorescent proteins useful for optical marking will evolve toward brighter, monomeric forms that are more diverse in color. We previously reported a new fluorescent protein from Aequorea macrodactyla, GFPxm, that exhibited many characteristics similar to wild-type green fluorescent protein (GFP).
Luo, W. X. +6 more
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Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]
, 2015 We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for ...
Leake, Mark C., Wollman, Adam J. M.
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