Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity [PDF]
Nature Communications, 2021 The link between gRNA sequence and Cas9 activity is well established but the mechanism underlying this relationship is not well understood. Here the authors show that gRNA sequence primarily influences activity by dictating the time it takes for Cas9 to ...
Eirik A Moreb, Michael D Lynch
exaly +5 more sources
Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities
Cell Discovery, 2023 Life science studies involving clustered regularly interspaced short palindromic repeat (CRISPR) editing generally apply the best-performing guide RNA (gRNA) for a gene of interest.
Heng Zhang +8 more
doaj +4 more sources
Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning
Nature Communications, 2021 High-quality gRNA activity data is needed for accurate on-target efficiency predictions. Here the authors generate activity data for over 10,000 gRNA and build a deep learning model CRISPRon for improved performance predictions.
Giulia Ilaria Corsi +2 more
exaly +3 more sources
Nucleic Acids Research, 2022 The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become a successful and promising technology for gene-editing.
Vasileios Konstantakos
exaly +2 more sources
Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing
Science, 2023 In Trypanosoma brucei, the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)–programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs ...
Shiheng Liu, , Xiaorun Li
exaly +2 more sources
Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
Nature Communications, 2022 The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs (gRNAs).
Stephan Riesenberg +2 more
exaly +2 more sources
Inducible expression of large gRNA arrays for multiplexed CRISPRai applications
Nature Communications, 2022 CRISPR gene activation and inhibition (CRISPRai) has become a powerful synthetic tool for influencing the expression of native genes for foundational studies, cellular reprograming, and metabolic engineering.
Lucie Studená +2 more
exaly +2 more sources
Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR–Cas12a gRNA switch
Nucleic Acids Research, 2021 CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease activity. One promising strategy involves linking nuclease activity to a cell's transcriptional state by engineering guide RNAs (gRNAs) to function only after ...
Chase Beisel
exaly +2 more sources
A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
Nature Communications, 2019 With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiting step in synthetic biology. Here, we report a gRNA-tRNA array for CRISPR-Cas9 (GTR-CRISPR) for multiplexed engineering of Saccharomyces cerevisiae ...
Yueping Zhang, Yiming Zhang, Shuobo Shi
exaly +2 more sources
Are the current gRNA ranking prediction algorithms useful for genome editing in plants?
PLoS ONE, 2020 Introducing a new trait into a crop through conventional breeding commonly takes decades, but recently developed genome sequence modification technology has the potential to accelerate this process. One of these new breeding technologies relies on an RNA-
Fatima Naim +7 more
doaj +2 more sources