Results 91 to 100 of about 60,004 (331)

Identification of small RNAs abundant in Burkholderia cenocepacia biofilms reveal putative regulators with a potential role in carbon and iron metabolism [PDF]

, 2017
Small RNAs play a regulatory role in many central metabolic processes of bacteria, as well as in developmental processes such as biofilm formation. Small RNAs of Burkholderia cenocepacia, an opportunistic pathogenic beta-proteobacterium, are to date not ...
Coenye, Tom, Kiekens, Sanne, Sass, Andrea   +2 more
core   +3 more sources

Interactions of mRNAs and gRNAs involved in trypanosome mitochondrial RNA editing: Structure probing of a gRNA bound to its cognate mRNA [PDF]

RNA, 2006
Expression of mitochondrial genes in Trypanosoma brucei requires RNA editing of its mRNA transcripts. During editing, uridylates are precisely inserted and deleted as directed by the gRNA template to create the protein open reading frame. This process involves the bimolecular interaction of the gRNA with its cognate pre-edited mRNA and the assembly of ...
Laura E, Yu, Donna J, Koslowsky
openaire   +2 more sources

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Visualization of Retroviral Gag-Genomic RNA Cellular Interactions Leading to Genome Encapsidation and Viral Assembly: An Overview

Viruses, 2022
Retroviruses must selectively recognize their unspliced RNA genome (gRNA) among abundant cellular and spliced viral RNAs to assemble into newly formed viral particles.
Serena Bernacchi
doaj   +1 more source

A CRISPR-Cas9 sex-ratio distortion system for genetic control. [PDF]

, 2016
Genetic control aims to reduce the ability of insect pest populations to cause harm via the release of modified insects. One strategy is to bias the reproductive sex ratio towards males so that a population decreases in size or is eliminated altogether ...
Bernardini, F, Crisanti, A, Galizi, R, Hammond, A, Kyrou, K, Nolan, T, O'Loughlin, SM, Papathanos, PA, Taxiarchi, C, Windbichler, N   +9 more
core   +3 more sources

The gRNA-miRNA-gRNA Ternary Cassette Combining CRISPR/Cas9 with RNAi Approach Strongly Inhibits Hepatitis B Virus Replication

Theranostics, 2017
The CRISPR/Cas9 system is a novel genome editing technology which has been successfully used to inhibit HBV replication. Here, we described a novel gRNA-microRNA (miRNA)-gRNA ternary cassette driven by a single U6 promoter. With an anti-HBV pri-miR31 mimic integrated between two HBV-specific gRNAs, both gRNAs could be separated from the long transcript
Wang, Jie, Chen, Ran, Zhang, Ruiyang, Ding, Shanlong, Zhang, Tianying, Yuan, Quan, Guan, Guiwen, Chen, Xiangmei, Zhang, Ting, Zhuang, Hui, Nunes, Frederick, Block, Timothy, Liu, Shuang, Duan, Zhongping, Xia, Ningshao, Xu, Zhongwei, Lu, Fengmin   +16 more
openaire   +2 more sources

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Nghiên cứu tạo phôi chuột knockout gene lep sử dụng kỹ thuật CRISPR/Cas9

Tạp chí Khoa học Đại học Cần Thơ, 2022
Kỹ thuật CRISPR/Cas 9 đang được sử dụng nhiều trong công nghệ chỉnh sửa gene. Trong nghiên cứu này, phôi chuột knockout gene Lep được tạo ra bằng cách vi tiêm plasmid có cấu trúc CRISPR/Cas9 hướng đến exon 3 của gene.
Âu Dương Tuyết Mai, Nguyễn Lê Trâm Anh, Nguyễn Thị Thanh Giang, Huỳnh Chí Thiện, Phạm Minh Chiến, Huỳnh Nguyễn Loan Anh, Nguyễn Đăng Quân   +6 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat

Frontiers in Genome Editing
BackgroundCRISPR/Cas9 technology has gained popularity due to its efficient, widely applicable, and relatively easy genome editing. Furthermore, the removal of regulation on site-directed nuclease1- (SDN1) and SDN2-developed products in many countries ...
Tushadri Singh, Tushadri Singh, H. M. Mamrutha, Rajender Singh, J. P. Jaiswal, Zeenat Wadhwa, Rakesh Kumar, Omvir Singh, O. P. Ahlawat, Ratan Tiwari   +9 more
doaj   +1 more source