Results 111 to 120 of about 60,004 (331)

Tendon Organoids Enable Functional Tendon Rejuvenation Through ALKBH5‐Dependent RNA Demethylation

Advanced Science, EarlyView.
FT organoids reverse the aged phenotype of tendon cells, reinstating a fetal‐like state. This breakthrough establishes a potent cell source for tendon tissue engineering, effectively advancing regenerative medicine. ABSTRACT Adult tendon injuries pose a major clinical challenge due to limited self‐repair capacity, resulting in suboptimal regeneration ...
Tian Qin, Aini Pan, Zhuoning Miao, Yanyan Zhao, Xianan Mo, Heng Sun, Chunmei Fan, Junyu Guo, Bingbing Wu, Weiliang Shen, Qiangqiang Zheng, Jun Lu, Xi Jiang, Zi Yin, Xiao Chen   +14 more
wiley   +1 more source

Co-delivery of Cas9 mRNA and guide RNAs for editing of LGMN gene represses breast cancer cell metastasis

Scientific Reports
Legumain (or asparagine endopeptidase/AEP) is a lysosomal cysteine endopeptidase associated with increased invasive and migratory behavior in a variety of cancers.
Yue Wang, Yatu Peng, Guanghui Zi, Jin Chen, Baowei Peng   +4 more
doaj   +1 more source

Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. [PDF]

, 2018
DNA methylation is an important epigenetic modification involved in gene regulation and transposable element silencing. Changes in DNA methylation can be heritable and, thus, can lead to the formation of stable epialleles. A well-characterized example of
Gallego-Bartolomé, Javier, Gardiner, Jason, Ghoshal, Basudev, Jacobsen, Steven E, Kuo, Hsuan Yu, Liu, Wanlu, Papikian, Ashot, Segal, David J, Zhao, Jenny Miao-Chi   +8 more
core   +2 more sources

Assembly of Mitochondrial Ribonucleoprotein Complexes Involves Specific Guide RNA (gRNA)-Binding Proteins and gRNA Domains but Does Not Require Preedited mRNA [PDF]

Molecular and Cellular Biology, 1994
RNA editing in kinetoplastids probably employs a macromolecular complex, the editosome, that is likely to include the guide RNAs (gRNAs) which specify the edited sequence. Specific ribonucleoprotein (RNP) complexes which form in vitro with gRNAs (H. U. Göringer, D. J. Koslowsky, T. H. Morales, and K. D. Stuart, Proc. Natl. Acad. Sci. USA, in press) are
L K, Read, H U, Göringer, K, Stuart
openaire   +2 more sources

Testicular mRNA‐LNP Delivery: A Novel Therapy for Genetic Spermatogenic Disorders

Advanced Science, EarlyView.
In the current study, we developed a novel therapy for genetic spermatogenic disorders. It was revealed that in vivo delivery of mRNA LNP3 could restore spermatogenesis in Msh5D486Y/D486Y and Maps KO mouse models with meiotic arrest. Notably, the offspring without genomic integration was born using ICSI derived from the rescue of Msh5D486Y/D486Y mouse ...
Chenwang Zhang, Nan Liang, Wenbo Li, Shuai Xu, Peng Li, Wanze Ni, Na Li, Sha Han, Ningjing Ou, Haowei Bai, Yuxiang Zhang, Furong Bai, Yifan Sun, Dewei Qian, Xinjie Bu, Erlei Zhi, Ruhui Tian, Yuhua Huang, Jingpeng Zhao, Fujun Zhao, Hao Chen, Zheng Li, Chencheng Yao   +22 more
wiley   +1 more source

A Golden Gate-based Protocol for Assembly of Multiplexed gRNA Expression Arrays for CRISPR/Cas9

Bio-Protocol, 2016
The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) has become the most broadly used and powerful tool for genome editing.
Johan Vad-Nielsen, Lin Lin , Kristopher Jensen, Anders Nielsen , Yonglun Luo   +4 more
doaj   +1 more source

Editosome Accessory Factors KREPB9 and KREPB10 in Trypanosoma brucei [PDF]

, 2012
Multiprotein complexes, called editosomes, catalyze the uridine insertion and deletion RNA editing that forms translatable mitochondrial mRNAs in kinetoplastid parasites.
Acestor, Achim Schnaufer, Alatortsev, Ammerman, Aphasizheva, Aphasizheva, Bailey, Borst, Carnes, Carnes, Carnes, Cruz-Reyes, Cruz-Reyes, Deschamps, Ernst, Ernst, Etheridge, Fisk, Guo, Guo, Guo, Hashimi, Igo, Igo, Inoue, Jason Carnes, Jensen, Kao, Kenneth Stuart, Koslowsky, Koslowsky, Lukes, Mattiacio, Mattiacio, Melissa Lerch, Militello, Nathalie Acestor, Niemann, Panigrahi, Panigrahi, Panigrahi, Panigrahi, Panigrahi, Panigrahi, Piller, Rigaut, Ryan, Sabatini, Schnaufer, Schnaufer, Seiwert, Simpson, Stuart, Trotter, Weng, Wirtz, Worthey, Xuemin Guo, Zhang   +58 more
core   +1 more source

gRNA Design: How Its Evolution Impacted on CRISPR/Cas9 Systems Refinement

Biomolecules, 2023
Over the past decade, genetic engineering has witnessed a revolution with the emergence of a relatively new genetic editing tool based on RNA-guided nucleases: the CRISPR/Cas9 system. Since the first report in 1987 and characterization in 2007 as a bacterial defense mechanism, this system has garnered immense interest and research attention.
Cristofer Motoche-Monar, Julián E. Ordoñez, Oscar Chang, Fernando A. Gonzales-Zubiate   +3 more
openaire   +3 more sources

Comprehensive Profiling of N6‐methyladnosine (m6A) Readouts Reveals Novel m6A Readers That Regulate Human Embryonic Stem Cell Differentiation

Advanced Science, EarlyView.
This research deciphers the m6A transcriptome by profiling its sites and functional readout effects: from mRNA stability, translation to alternative splicing, across five different cell types. Machine learning model identifies novel m6A‐binding proteins DDX6 and FXR2 and novel m6A reader proteins FUBP3 and L1TD1.
Zhou Huang, Rucong Liu, Zibaguli Wubulikasimu, Wanqing Zhao, Jiaqi Huang, Jiaxuan Wang, Tianyuan Zhang, Rui Fan, Wei Kong, Qinghua Cui, Yang Li, Yuan Zhou   +11 more
wiley   +1 more source

CRISPR/Cas9-induced (CTG⋅CAG)n repeat instability in the myotonic dystrophy type 1 locus: implications for therapeutic genome editing [PDF]

, 2017
Myotonic dystrophy type 1 (DM1) is caused by (CTG⋅CAG)n-repeat expansion within the DMPK gene and thought to be mediated by a toxic RNA gain of function.
André, Laurène M., Cumming, Sarah A., Furling, Denis, Gourdon, Geneviève, Monckton, Darren G., Mouly, Vincent, van Agtmaal, Ellen L., van den Broek, Walther J.A.A., van Kessel, Ingeborg D.G., Wansink, Derick G., Wieringa, Bé, Willemse, Marieke   +11 more
core   +4 more sources