Results 121 to 130 of about 60,004 (331)

BioLOGIC: Logic Orthogonal gRNA-Implemented Circuits

, 2015
Poster for iGEM 2015 Giant Jamboree by the Ecole Polytechnique Fédérale de Lausanne (EPFL) team.
Cuillery, Emilie, Heng, Joseph, Jacquot, Vincent, Malsot, Paola, Meli, Rocco, Pulver, Cyril, Sarfatis, Ari, Steiner, Loïc, Steininger, Victor, Thouvenin, Grégoire, Uran, Axel, van Tiel, Nina   +11 more
openaire   +1 more source

Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes [PDF]

ACS Synthetic Biology, 2018
Lineage tracking delivers essential quantitative insight into dynamic, probabilistic cellular processes, such as somatic tumor evolution and differentiation. Methods for high diversity lineage quantitation rely on sequencing a population of DNA barcodes. However, manipulation of specific individual lineages is not possible with this approach.
Aziz M. Al’Khafaji, Daniel Deatherage, Amy Brock   +2 more
openaire   +2 more sources

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

An improved CRISPRi system in Pichia pastoris

Synthetic and Systems Biotechnology, 2023
CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains.
Shujing Qiao, Fan Bai, Peng Cai, Yongjin J. Zhou, Lun Yao   +4 more
doaj   +1 more source

CasOT: a genome-wide Cas9/gRNA off-target searching tool [PDF]

Bioinformatics, 2014
Abstract Summary: The CRISPR/Cas or Cas9/guide RNA system is a newly developed, easily engineered and highly effective tool for gene targeting; it has considerable off-target effects in cultured human cells and in several organisms. However, the Cas9/guide RNA target site is too short for existing alignment tools to exhaustively and ...
An, Xiao, Zhenchao, Cheng, Lei, Kong, Zuoyan, Zhu, Shuo, Lin, Ge, Gao, Bo, Zhang   +6 more
openaire   +2 more sources

The Disordered Region of ASXL1 Acts as an Auto‐Regulator Through Condensation

Advanced Science, EarlyView.
ASXL1's long IDR encodes an electrostatic “basic platform + acidic brake” that autoregulates condensation. Truncation at a clinical hotspot lifts this brake, forming condensates that retarget BRD2, remodel local chromatin accessibility, and impair neutrophil maturation.
Xiao Fang, Qiwei Li, Wenqing Zhang
wiley   +1 more source

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]

, 2015
Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F, Kaulich, Manuel, Lee, Yeon J, Lönn, Peter, Meade, Bryan R, Springer, Aaron D   +5 more
core   +1 more source

Hazelnut allergome overview and Cor a gRNAs identification

BMC Plant Biology
Corylus species (hazelnuts) are a valuable source of nutrients and are widely consumed worldwide. Nevertheless, Corylus avellana (Cor a) contains 13 allergens (Cor a 1, Cor a 2, Cor a 6, Cor a 8, Cor a 9, Cor a 10, Cor a 11, Cor a 12, Cor a 13, Cor a 14, Cor a 15, Cor a 16, and Cor a TLP) that have been deposited into the official database (WHO/IUIS ...
Amoroso, Ciro Gianmaria, Andolfo, Giuseppe   +1 more
openaire   +4 more sources

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays

PLOS ONE, 2018
The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immunity in bacteria. This system has been adopted for numerous genome engineering applications in research and recently, therapeutics. The CRISPR/Cas9 system has been largely implemented by delivery of Cas9 as protein, RNA, or plasmid along with a chimeric ...
Morito Kurata, Natalie K Wolf, Walker S Lahr, Madison T Weg, Mitchell G Kluesner, Samantha Lee, Kai Hui, Masano Shiraiwa, Beau R Webber, Branden S Moriarity   +9 more
openaire   +4 more sources

MAGIC: Mosaic Analysis by gRNA-Induced Crossing-over [PDF]

, 2020
AbstractMosaic animals have provided the platform for many fundamental discoveries in developmental biology, cell biology, and other fields. Techniques to produce mosaic animals by mitotic recombination have been extensively developed inDrosophila melanogasterbut are less common for other laboratory organisms.
Sarah E. Allen, Gabriel T. Koreman, Ankita Sarkar, Bei Wang, Mariana F. Wolfner, Chun Han   +5 more
openaire   +1 more source