Results 131 to 140 of about 60,004 (331)
Pathogenic Role of FGFR3 Autoantibodies in Small Fiber Neuropathy
Advanced Science, EarlyView.Autoantibodies against fibroblast growth factor receptor 3 (FGFR3) are identified as pathogenic drivers of pain in small fiber neuropathy. By binding to sensory neurons in dorsal root ganglia, FGFR3 autoantibodies activate MAPK signaling and induce hyperexcitability and mechanical hypersensitivity, establishing FGFR3 autoantibodies as a therapeutic ...
Lyuba Y. Salih +12 more
wiley +1 more source
mSphere, 2018 Cryptococcus neoformans and related species are encapsulated basidiomycetous fungi that cause meningoencephalitis in individuals with immune deficiency.
Ping Wang
doaj +1 more source
The CaMKII/NMDA receptor complex controls hippocampal synaptic transmission by kinase-dependent and independent mechanisms. [PDF]
, 2018 CaMKII is one of the most studied synaptic proteins, but many critical issues regarding its role in synaptic function remain unresolved. Using a CRISPR-based system to delete CaMKII and replace it with mutated forms in single neurons, we have rigorously ...
Asensio, Cedric S +8 more
core +2 more sources
Versatile CRISPR‐Cas Tools for Gene Regulation in Zebrafish via an Enhanced Q Binary System
Advanced Science, EarlyView.This study introduces CRISPR‐Q, a transgenic CRISPR‐Cas system leveraging the QFvpr/QUAS binary expression platform in zebrafish. CRISPR‐Q overcomes previous challenges in achieving stable and efficient gene regulation. By enabling precise spatiotemporal control of transcript knockdown (CRISPR‐QKD) and gene activation (CRISPR‐Qa), it provides a ...
Miaoyuan Shi +13 more
wiley +1 more source
BMC Research Notes, 2017 Objective Recombinant adeno-associated virus (AAV)-based vectors are characterized by their robust and safe transgene delivery. The CRISPR/Cas9 and guide RNA (gRNA) system present a promising genome-editing platform, and a recent development of a shorter
Salma G. Morsy +8 more
doaj +1 more source
CasX GFP-Targeting gRNA IVT v1
, 2019 This protocol explains how to transcribe gRNA in vitro.
Connor Tsuchida, Liz O'Brien
openaire +1 more source
Advanced Science, EarlyView.Using human induced pluripotent stem cells (hiPSCs)‐derived neuronal model, Tian and colleagues reveal that voltage‐gated calcium channels Cav1.2 and Cav1.3, and their mediated calcium ion influx, are essential for early morphogenesis of human neuronal development, while ECEL1 underlies human neuronal functional developmental maturation through CALM3 ...
Yue Tian +5 more
wiley +1 more source
The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing.
PLoS ONE, 2010 Mitochondrial mRNA editing in Trypanosoma brucei requires the specific interaction of a guide RNA with its cognate mRNA. Hundreds of gRNAs are involved in the editing process, each needing to target their specific editing domain within the target message.
Larissa Reifur +4 more
doaj +1 more source
CRISPR-Cas9-mediated functional dissection of 3'-UTRs. [PDF]
, 2017 Many studies using reporter assays have demonstrated that 3' untranslated regions (3'-UTRs) regulate gene expression by controlling mRNA stability and translation.
Ahituv, Nadav +6 more
core +1 more source
Advanced Science, EarlyView.This study discovered a new pathway that tells fruit flies when to stop eating. It found that rising blood sugar (fructose) is detected by a sensor called GR43a. This triggers a chain reaction involving the satiety signal sulfakinin and its receptor, ultimately activating a final satiety signal, ILP5.
Hong‐Fei Li +7 more
wiley +1 more source