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Methods in Molecular Biology, 2019
Gene editing has great therapeutic impact, being of interest for many scientists worldwide. Clustered regularly interspaced short palindromic repeats (CRISPR) technology has been adapted for gene editing to serve as an efficient, rapid, and cost-effective tool.
Nastassia Knödlseder, Marc Guell
exaly +3 more sources
Gene editing has great therapeutic impact, being of interest for many scientists worldwide. Clustered regularly interspaced short palindromic repeats (CRISPR) technology has been adapted for gene editing to serve as an efficient, rapid, and cost-effective tool.
Nastassia Knödlseder, Marc Guell
exaly +3 more sources
Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing [PDF]
Science, 2023 In Trypanosoma brucei, the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs ...
Ana Lúcia Alvarez Cabrera +2 more
exaly +2 more sources
Synthetic gRNA/Cas9 Ribonucleoprotein Inhibits HIV Reactivation and Replication
Viruses, 2022 The current antiretroviral therapy (ART) for human immunodeficiency virus (HIV) can halt viral replication but cannot eradicate HIV infection because proviral DNA integrated into the host genome remains genetically silent in reservoir cells and is ...
Shunbin Ning +2 more
exaly +2 more sources
gRNA-transient expression system for simplified gRNA delivery in CRISPR/Cas9 genome editing
Journal of Bioscience and Bioengineering, 2019The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) system is one of the most powerful tools for genome engineering. However, some of the steps are laborious, reducing its usability. In this study, we have developed a simplified method, called the guide RNA-transient expression system ...
Farhana Easmin +5 more
openaire +2 more sources
Metabolic Engineering, 2021
Saccharomyces cerevisiae is a versatile microbial platform to build synthetic metabolic pathways for production of diverse chemicals. To expedite the construction of complex metabolic pathways by multiplex CRISPR-Cas9 genome-edit, eight desirable intergenic loci, located adjacent to highly expressed genes selected from top 100 expressers, were ...
Sihyun Baek +5 more
openaire +2 more sources
Saccharomyces cerevisiae is a versatile microbial platform to build synthetic metabolic pathways for production of diverse chemicals. To expedite the construction of complex metabolic pathways by multiplex CRISPR-Cas9 genome-edit, eight desirable intergenic loci, located adjacent to highly expressed genes selected from top 100 expressers, were ...
Sihyun Baek +5 more
openaire +2 more sources
Cycles of progressive realignment of gRNA with mRNA in RNA editing
Cell, 1991We characterized numerous partially edited NADH dehydrogenase 7 and ATPase 6 cDNAs. Most of these have a stretch of incompletely edited sequence at the junction of mature and unedited sequences. The characteristics of the junctions suggest editing of sites multiple times and that editing within each junction does not proceed precisely 3' to 5 ...
D J, Koslowsky +3 more
openaire +2 more sources
gRNA Design: How Its Evolution Impacted on CRISPR/Cas9 Systems Refinement
Biomolecules, 2023Fernando Gonzales +2 more
exaly
PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies
Cells, 2022Xiaohan Yang +2 more
exaly