Results 21 to 30 of about 60,004 (331)

PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System

Bio-Protocol, 2021
Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable
Naim Hassan, Farhana Easmin, Keisuke Ekino , Satoshi Harashima   +3 more
doaj   +1 more source

The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments

Scientific Reports, 2023
Gene editing is a promising alternative to traditional breeding for the generation of new mushroom strains. However, the current approach frequently uses Cas9-plasmid DNA to facilitate mushroom gene editing, which can leave residual foreign DNA in the ...
Hye-Hyun Eom, Yeon-Jae Choi, R. Nandre, Hui-Gang Han, S. Kim, Minseek Kim, Y. Oh, T. Nakazawa, Y. Honda, H. Ro   +9 more
semanticscholar   +1 more source

CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context

Nature Communications, 2022
A major challenge of CRISPR/Cas9-mediated genome engineering is that not all guide RNAs (gRNAs) cleave the DNA efficiently. Although the heterogeneity of gRNA activity is well recognized, the current understanding of how CRISPR/Cas9 activity is regulated
G. Corsi, Kunli Qu, F. Alkan, Xiaoguang Pan, Yonglun Luo, J. Gorodkin   +5 more
semanticscholar   +1 more source

Guide RNA Repertoires in the Main Lineages of Trypanosoma cruzi: High Diversity and Variable Redundancy Among Strains

Frontiers in Cellular and Infection Microbiology, 2021
Trypanosoma cruzi, as other kinetoplastids, has a complex mechanism of editing of mitochondrial mRNAs that requires guide RNAs (gRNAs) coded in DNA minicircles in the kinetoplast. There are many variations on this mechanism among species.
Fanny Rusman, Noelia Floridia-Yapur, Nicolás Tomasini, Patricio Diosque   +3 more
doaj   +1 more source

C-SVR Crispr: Prediction of CRISPR/Cas12 guideRNA activity using deep learning models

Alexandria Engineering Journal, 2021
Clustered regularly interspaced short palindromic repeat (CRISPR) technology is the most important tool in gene editing, it can be used to target any gene using guide RNA and Cas enzyme, one limitation of CRISPR systems is low guide RNA (gRNA) activity ...
Zubaida Sa'id Ameen, Mehmet Ozsoz, Auwalu Saleh Mubarak, Fadi Al Turjman, Sertan Serte   +4 more
doaj   +1 more source

Emergence of Compensatory Mutations Reveals the Importance of Electrostatic Interactions between HIV-1 Integrase and Genomic RNA

mBio, 2022
HIV-1 integrase (IN) has a noncatalytic function in virion maturation through its binding to the viral RNA genome (gRNA). Class II IN substitutions inhibit IN-gRNA binding and result in the formation of virions with aberrant morphologies marked by ...
Christian Shema Mugisha, Tung Dinh, Abhishek Kumar, Kasyap Tenneti, Jenna E. Eschbach, Keanu Davis, Robert Gifford, Mamuka Kvaratskhelia, Sebla B. Kutluay   +8 more
doaj   +1 more source

Creating a tobacco line with a weaker antifeedant property against colorado potato beetle

Биотехнология и селекция растений, 2020
Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina, A. V. Spaselnikova, A. A. Egorova, E. V. Kolosovskaya, D. V. Domrachev, A. V. Romanova, S. R. Tumanyan, S. Chamas, J. Kumlehn, I. M. Dubovskiy, S. V. Gerasimova   +10 more
doaj   +1 more source

Trypanosome RNA helicase KREH2 differentially controls non-canonical editing and putative repressive structure via a novel proposed ‘bifunctional’ gRNA in mRNA A6

Nucleic Acids Research, 2023
U-insertion/deletion (U-indel) RNA editing in trypanosome mitochondria is directed by guide RNAs (gRNAs). This editing may developmentally control respiration in bloodstream forms (BSF) and insect procyclic forms (PCF).
Joshua Meehan, Suzanne M. McDermott, A. Ivens, Zachary Goodall, Zihao Chen, Zihao Yu, J. Woo, Tyler Rodshagen, Laura McCleskey, Rebecca Sechrist, K. Stuart, Lanying Zeng, Silvi Rouskin, N. Savill, A. Schnaufer, Xiuren Zhang, J. Cruz-Reyes   +16 more
semanticscholar   +1 more source

Assessment of anti-HIV-1 guide RNA efficacy in cells containing the viral target sequence, corresponding gRNA, and CRISPR/Cas9

Frontiers in Genome Editing, 2023
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 gene editing system has been shown to be effective at inhibiting human immunodeficiency virus type 1 (HIV-1).
A. Allen, C. Chung, S. Worrell, G. Nwaozo, R. Madrid, A. Mele, W. Dampier, M. Nonnemacher, B. Wigdahl   +8 more
semanticscholar   +1 more source

Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA

Plant Physiology, 2022
As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits.
W. P. Bewg, S. Harding, N. Engle, B. Vaidya, R. Zhou, J. Reeves, Thomas W. Horn, N. Joshee, J. Jenkins, S. Shu, K. Barry, Y. Yoshinaga, J. Grimwood, Robert J. Schmitz, J. Schmutz, T. Tschaplinski, Chung-Jui Tsai   +16 more
semanticscholar   +1 more source