Results 31 to 40 of about 60,004 (331)
Viruses, 2022 The SARS-CoV-2 infection generates up to nine different sub-genomic mRNAs (sgRNAs), in addition to the genomic RNA (gRNA). The 5′UTR of each viral mRNA shares the first 75 nucleotides (nt.) at their 5′end, called the leader, but differentiates by a ...
L. Condé +3 more
semanticscholar +1 more source
Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]
, 2020 Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu +2 more
core +2 more sources
Rational gRNA design based on transcription factor binding data [PDF]
Synthetic Biology, 2021 Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has become a standard tool in many genome engineering endeavors. The endonuclease-deficient version of Cas9 (dCas9) is also a powerful programmable tool for gene regulation.
David Bergenholm +5 more
openaire +2 more sources
Indonesian Biomedical Journal, 2023 BACKGROUND: Glutathione S-transferase Mu-1 (GSTM1) is known to undergo polymorphism and plays role in drug metabolism including Paclitaxel (PTX), the first-line chemotherapy for breast cancer.
Emilia Vivi Arsita +2 more
doaj +1 more source
Bioinform., 2022 MOTIVATION The CRISPR/Cas9 system is widely used for genome editing. The editing efficiency of CRISPR/Cas9 is mainly determined by the guide RNA (gRNA).
Yuhao Chen, Xiaowei Wang
semanticscholar +1 more source
Frontiers in Plant Science, 2023 CRISPR/Cas9-based genome editing system is a powerful tool for plant genetic improvement. However, the variable efficiency of guide RNA(s) (gRNA) represents a key limiting factor that hampers the broad application of the CRISPR/Cas9 system in crop ...
Zhibo Wang +6 more
doaj +1 more source
Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
Genome Biology, 2018 CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA.
Yu Hong +4 more
doaj +1 more source
PLoS Genetics, 2023 Retroviruses and closely related LTR retrotransposons export full-length, unspliced genomic RNA (gRNA) for packaging into virions and to serve as the mRNA encoding GAG and POL polyproteins.
Bing Sun +3 more
doaj +1 more source
Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import
Scientific Reports, 2022 Achieving CRISPR Cas9-based manipulation of mitochondrial DNA (mtDNA) has been a long-standing goal and would be of great relevance for disease modeling and for clinical applications.
Ludwig Schmiderer +4 more
semanticscholar +1 more source
High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa). [PDF]
, 2018 CRISPR/Cas9 is a transformative tool for making targeted genetic alterations. In plants, high mutation efficiencies have been reported in primary transformants. However, many of the mutations analyzed were somatic and therefore not heritable.
Bertier, Lien D +5 more
core +2 more sources