Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
Bio-Protocol, 2020 Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored ...
Martin Wegner +2 more
doaj +1 more source
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling. [PDF]
, 2020 The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of ...
Aris, Kevin D Palacio +5 more
core +3 more sources
The insect-phase gRNA transcriptome in Trypanosoma brucei [PDF]
Nucleic Acids Research, 2013 One of the most striking examples of small RNA regulation of gene expression is the process of RNA editing in the mitochondria of trypanosomes. In these parasites, RNA editing involves extensive uridylate insertions and deletions within most of the mitochondrial messenger RNAs (mRNAs).
Koslowsky, Donna +4 more
openaire +2 more sources
PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies
Cells, 2022 Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps.
Guoliang Yuan +4 more
semanticscholar +1 more source
Development of Target-Specific gRNAs for CRISPR-Cas9 Mediated Antifungal Resistance in Sweet Pepper [PDF]
BIO Web of ConferencesUsing CRISPR-Cas9, the gRNA for antifungal resistance to mildew diseases in Pepper species, especially Capsicum annuum, is designed. The Downey Mildew Resistance (DMR), and Enhanced Disease Resistance (EDR) genes were chosen, and the target-specific gRNA
M Sedarshini +5 more
doaj +1 more source
In Planta Processing of the SpCas9–gRNA Complex [PDF]
Plant and Cell Physiology, 2017 In CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)-mediated genome editing in plants, Streptococcus pyogenes Cas9 (SpCas9) protein and the required guide RNA (gRNA) are, in most cases, expressed from a stably integrated transgene.
Mikami, Masafumi +2 more
openaire +2 more sources
CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis [PDF]
, 2016 Retinoblastoma is a pediatric eye tumor in which bi-allelic inactivation of the Retinoblastoma 1 (RB1) gene is the initiating genetic lesion. Although recently curative rates of retinoblastoma have increased, there are at this time no molecular targeted ...
Boel, Annekatrien +12 more
core +2 more sources
CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding [PDF]
, 2020 Open Access Journal; Published online: 31 Jan 2019Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids.
Britt, A. +5 more
core +1 more source
Comparative structural and dynamics study of free and gRNA-bound FnCas9 and SpCas9 proteins
bioRxiv, 2022 The introduction of CRISPR/Cas9 based gene editing has greatly accelerated therapeutic genome editing. However, the off-target DNA cleavage by CRISPR/Cas9 protein hampers its clinical translation, thereby hindering its widespread use as a programmable ...
G. Panda, Arjun Ray
semanticscholar +1 more source