CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]
, 2019 The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad +8 more
core +1 more source
PLOS ONE, 2018 Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time. A significant advantage of CRISPR over previous methods is its tremendous adaptability due to its bipartite nature. Cas9 or its engineered variants define the molecular effect, while short gRNAs
Christopher T. Breunig +8 more
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A mRNA determinant of gRNA-directed kinetoplastid editing [PDF]
Nucleic Acids Research, 2001 Several mitochondrial mRNAs of the kinetoplastid protozoa do not encode a functional open reading frame until they have been edited through the addition or deletion of U nucleotides at specific sites. Genetic information specifying the location and extent of editing is present on guide RNAs (gRNAs).
A L, Kabb +3 more
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A multiplex guide RNA expression system and its efficacy for plant genome engineering
Plant Methods, 2020 Background The Streptococcus pyogenes CRISPR system is composed of a Cas9 endonuclease (SpCas9) and a single-stranded guide RNA (gRNA) harboring a target-specific sequence.
Youngbin Oh +3 more
doaj +1 more source
CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury
PLoS Genetics, 2021 Zebrafish exhibit robust regeneration following spinal cord injury, promoted by macrophages that control post-injury inflammation. However, the mechanistic basis of how macrophages regulate regeneration is poorly understood.
Marcus Keatinge +9 more
semanticscholar +1 more source
Gene repression via multiplex gRNA strategy in Y. lipolytica [PDF]
Microbial Cell Factories, 2018 The oleaginous yeast Yarrowia lipolytica is a promising microbial cell factory due to their biochemical characteristics and native capacity to accumulate lipid-based chemicals. To create heterogenous biosynthesis pathway and manipulate metabolic flux in Y.
Jin-lai Zhang +7 more
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Scientific Reports, 2021 Rapid and accurate species diagnosis accelerates performance in numerous biological fields and associated areas. However, morphology-based species taxonomy/identification might hinder study and lead to ambiguous results.
Kittisak Buddhachat +5 more
doaj +1 more source
Synthetic Circular gRNA Mediated Biological Function of CRISPR-(d)Cas9 System
Frontiers in Cell and Developmental Biology, 2022 Ever since the gene editing function was discovered in the CRISPR-Cas9 system, numerous applications and utilities were investigated in order to apply this technique to medical use.
Mingxia Wang +3 more
semanticscholar +1 more source
CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]
, 2018 The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad +7 more
core +1 more source
Differential Effects of Arginine Methylation on RBP16 mRNA Binding, Guide RNA (gRNA) Binding, and gRNA-containing Ribonucleoprotein Complex (gRNP) Formation [PDF]
Journal of Biological Chemistry, 2007 Mitochondrial gene expression in Trypanosoma brucei involves the coordination of multiple events including polycistronic transcript cleavage, polyadenylation, RNA stability, and RNA editing. Arg methylation of RNA binding proteins has the potential to influence many of these processes via regulation of protein-protein and protein-RNA interactions. Here
Christopher C, Goulah, Laurie K, Read
openaire +2 more sources