Results 61 to 70 of about 60,004 (331)

Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System

Bio-Protocol, 2014
RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for genome editing in many organisms including plant and crop ...
Kabin Xie, Bastian Minkenberg, Yinong Yang   +2 more
doaj   +1 more source

Programmable RNA base editing with a single gRNA-free enzyme

bioRxiv, 2021
Programmable RNA editing enables rewriting gene expression without changing genome sequences. Current tools for specific RNA editing dependent on the assembly of guide RNA into an RNA/protein complex, causing delivery barrier and low editing efficiency ...
Wenjian Han, Wendi Huang, Miaowei Mao, Tong Wei, Yan-Bo Ye, Zefeng Wang   +5 more
semanticscholar   +1 more source

An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]

, 2018
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J, Chan, Yingleong, Chavez, Alejandro, Church, George M, Church, George M, Collins, James J, Davidsohn, Noah, Ebrahimkhani, Mo R, Guo, Xiaoge, Kiani, Samira, Kuo, Chih-Chung, Lance-Byrne, Alissa, Lewis, Nathan E, Lim, Elaine T, Menn, David, Milanova, Denitsa, Pradhan, Swechchha, Sharma, Sumana, Tung, Angela, Tuttle, Marcelle, Yeo, Nan Cher   +20 more
core   +1 more source

RNA-Binding Domains of Heterologous Viral Proteins Substituted for Basic Residues in the RSV Gag NC Domain Restore Specific Packaging of Genomic RNA

Viruses, 2020
The Rous sarcoma virus Gag polyprotein transiently traffics through the nucleus, which is required for efficient incorporation of the viral genomic RNA (gRNA) into virus particles.
Breanna L. Rice, Timothy L. Lochmann, Leslie J. Parent   +2 more
doaj   +1 more source

In Vivo Inhibition of Marek’s Disease Virus in Transgenic Chickens Expressing Cas9 and gRNA against ICP4

Microorganisms, 2021
Marek’s disease (MD), caused by MD herpesvirus (MDV), is an economically important disease in chickens. The efficacy of the existing vaccines against evolving virulent stains may become limited and necessitates the development of novel antiviral ...
A. Challagulla, K. Jenkins, T. O'Neil, Shunning Shi, K. Morris, T. Wise, Prasad N. Paradkar, M. Tizard, T. Doran, K. Schat   +9 more
semanticscholar   +1 more source

Differential in vitro infection of neural cells by astroviruses [PDF]

, 2019
Encephalitis remains a diagnostic conundrum in humans as over 50% of cases are managed without the identification of an etiology. Astroviruses have been detected from the central nervous system of mammals in association with disease, suggesting that this
Janowski, Andrew B, Klein, Robyn S, Wang, David   +2 more
core   +2 more sources

A Customizable Protocol for String Assembly gRNA Cloning (STAgR) [PDF]

Journal of Visualized Experiments, 2018
The bacterial CRISPR/Cas9 system has substantially increased methodological options for life scientists. Due to its utilization, genetic and genomic engineering became applicable to a large range of systems. Moreover, many transcriptional and epigenomic engineering approaches are now generally feasible for the first time.
Breunig, C., Neuner, A.M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S.H.   +5 more
openaire   +3 more sources

Optimized Plasmid Construction Strategy for Cas9

Cellular Physiology and Biochemistry, 2018
Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications.
Jianyong  Xu, Wenlei Li, MD. Munnaf  Hossen, Yuning Jia, Lingyun Li, Zhong Huang   +5 more
doaj   +1 more source

CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering

Molecular Systems Biology, 2020
Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target ...
Daniel Paolo Anderson, Henry James Benns, Edward William Tate, Matthew Andrew Child   +3 more
doaj   +1 more source

Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions. [PDF]

, 2017
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics.
A Baryshnikova, A Baryshnikova, A DeLean, Aaron N Chang, AE Briner, Alex N Beckett, Alex Thomas, AM Kabadi, Amanda Birmingham, Ana Bojorquez-Gomez, ASL Wong, Assen Roguev, B Vogelstein, C Laufer, Chih-Chung Kuo, CJ Lord, Dan Du, Daniel Pekin, Dongxin Zhao, F Lori, H Xu, J Shi, Jason F Kreisberg, JD Storey, Jens Luebeck, JG Doench, John Paul Shen, Katherine Licon, Kristin Klepper, KS Pollard, Kyle Salinas Sanchez, L Avery, LA Gilbert, Lei Qi, M Costanzo, M Martin, MC Bassik, MS Neshat, Nathan E Lewis, NE Sanjana, Nevan Krogan, NJ Krogan, P Mali, P Mali, Prashant Mali, R Chari, R Mani, R Srivas, Roman Sasik, S Bandyopadhyay, SR Collins, T Hart, T Horn, Trey Ideker, VG Tusher, Y Benjamini, Y Dang   +56 more
core   +1 more source