Results 71 to 80 of about 60,004 (331)

Photoswitchable gRNAs for Spatiotemporally Controlled CRISPR-Cas-Based Genomic Regulation [PDF]

ACS Central Science, 2020
The recently discovered CRISPR-Cas gene editing system and its derivatives have found numerous applications in fundamental biology research and pharmaceutical sciences. The need for precise external control over the gene editing and regulatory events has driven the development of inducible CRISPR-Cas systems. While most of the light-controllable CRISPR-
Elena V. Moroz-Omori, Dwiantari Satyapertiwi, Marie-Christine Ramel, Håkon Høgset, Ilona K. Sunyovszki, Ziqian Liu, Jonathan P. Wojciechowski, Yueyun Zhang, Christopher L. Grigsby, Liliana Brito, Laurence Bugeon, Margaret J. Dallman, Molly M. Stevens   +12 more
openaire   +5 more sources

Comparative Analysis of Cell Dilution Assays for Single-Cell Colony Formation in CRISPR-Mediated Knockout Screening

Cyprus Journal of Medical Sciences
BACKGROUND/AIMS: The ribonucleic acid (RNA)-guided Cas9 nuclease from the microbial clustered regularly interspaced palindromic repeats (CRISPR) immune system enables genome editing in eukaryotic cells by using a 20-nucleotide target sequence guide RNA ...
Hülya Dönmez, Bahadır Batar, Burhan Turgut   +2 more
doaj   +1 more source

Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction

BMC Genomics
Ensuring sufficient gRNA transcript levels is critical for obtaining optimal CRISPR-Cas9 gene editing efficiency. The standard gRNA scaffold contains a sequence of four thymine nucleotides (4T), which is known to inhibit transcription from Pol III ...
Y. Chey, Luke Gierus, Caleb Lushington, Jayshen Arudkumar, Ashleigh B Geiger, L. G. Staker, Louise J. Robertson, Chandran Pfitzner, Jesse G Kennedy, Ryan H B Lee, Gelshan I Godahewa, Fatwa Adikusuma, Paul Q. Thomas   +12 more
semanticscholar   +1 more source

Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo

Scientific Reports, 2021
C9ORF72 GGGGCC repeat expansion is the most common genetic cause for amyotrophic lateral sclerosis and frontotemporal dementia, which generates abnormal DNA and RNA structures and produces toxic proteins.
Xuejiao Piao, Dawei Meng, Xue Zhang, Qiang Song, Hailong Lv, Yichang Jia   +5 more
semanticscholar   +1 more source

Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. [PDF]

, 2017
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase,
Bai, Haipeng, Huang, Haigen, Li, Song, Lin, Shuo, Lu, Xiaochan, Qin, Wei, Xu, Jason, Zhang, Yihan   +7 more
core   +1 more source

CRISPR gRNAs cloning v1

, 2022
This protocol details the procedure of CRISPR gRNAs cloning.
openaire   +1 more source

On the Selective Packaging of Genomic RNA by HIV-1

Viruses, 2016
Like other retroviruses, human immunodeficiency virus type 1 (HIV-1) selectively packages genomic RNA (gRNA) during virus assembly. However, in the absence of the gRNA, cellular messenger RNAs (mRNAs) are packaged.
Mauricio Comas-Garcia, Sean R. Davis, Alan Rein   +2 more
doaj   +1 more source

Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators

Nature Communications
Small extracellular vesicles (sEVs) are important intercellular information transmitters in various biological contexts, but their release processes remain poorly understood.
Koki Kunitake, T. Mizuno, Kazuki Hattori, Chitose Oneyama, M. Kamiya, Sadao Ota, Y. Urano, Ryosuke Kojima   +7 more
semanticscholar   +1 more source

CROPSR: an automated platform for complex genome-wide CRISPR gRNA design and validation

BMC Bioinformatics, 2021
CRISPR/Cas9 technology has become an important tool to generate targeted, highly specific genome mutations. The technology has great potential for crop improvement, as crop genomes are tailored to optimize specific traits over generations of breeding ...
Hans Müller Paul, Dave D. Istanto, Jacob R Heldenbrand, M. Hudson   +3 more
semanticscholar   +1 more source

Intronic gRNAs for the Construction of Minimal Gene Drive Systems

Frontiers in Bioengineering and Biotechnology, 2022
Gene drives are promising tools for the genetic control of insect vector or pest populations. CRISPR-based gene drives are generally highly complex synthetic constructs consisting of multiple transgenes and their respective regulatory elements. This complicates the generation of new gene drives and the testing of the behavior of their constituent ...
Nash, A, Paolo, C, Hoermann, A, Papathanos, P, Windbichler, N   +4 more
openaire   +5 more sources