Developing, optimizing and using FRET-based biosensors to elucidate G protein signaling [PDF]
, 2020 The aim of the research presented in this thesis was to gain a better understanding of how intracellular signaling by GPCRs is regulated. We used fluorescence microscopy to study GPCR signaling in live cells.
Mastop, M.
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Leukemia-Associated Rho Guanine Nucleotide Exchange Factor Promotes G q-Coupled Activation of RhoA [PDF]
, 2002 Leukemia-associated Rho guanine-nucleotide exchange factor (LARG) belongs to the subfamily of Dbl homology RhoGEF proteins (including p115 RhoGEF and PDZ-RhoGEF) that possess amino-terminal regulator of G protein signaling (RGS) boxes also found within ...
Booden, M. A. +2 more
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Journal of Biological Chemistry, 1992 The ADP-ribosyl moiety of NAD was transferred to a 40-kDa protein when rat liver nuclei were incubated with pertussis toxin. The 40-kDa substrate in the nuclei displayed unique properties as follows, some of which were apparently distinct from those ...
Y. Takei +3 more
semanticscholar +1 more source
Regulation of blood platelet function by nitric oxide [PDF]
, 2013 Upon vascular injury, platelets instantly adhere to the exposed extracellular matrix resulting in platelet activation and aggregation to form a haemostatic plug.
Wake, Jonathan D.
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Activation of G(12)/G(13) results in shape change and Rho/Rho-kinase-mediated myosin light chain phosphorylation in mouse platelets [PDF]
, 1999 Platelets respond to various stimuli with rapid changes in shape followed by aggregation and secretion of their granule contents. Platelets lacking the alpha-subunit of the heterotrimeric G protein G(q) do not aggregate and degranulate but still undergo ...
Klages, Birgit +4 more
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Serotonin 5-HT4 receptor boosts functional maturation of dendritic spines via RhoA-dependent control of F-actin [PDF]
, 2020 Activity-dependent remodeling of excitatory connections underpins memory formation in the brain. Serotonin receptors are known to contribute to such remodeling, yet the underlying molecular machinery remains poorly understood.
Abdel-Galil, D +17 more
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Journal of Biological Chemistry, 1987 The role of Mg2+ in the GTP hydrolytic cycle was investigated by using purified subunits (G alpha and G beta, gamma) of the GTP-binding protein isolated from Bufo marinus rod outer segments (ROS). Mg2+ markedly stimulated the rate of GTP and guanosine-5'-
A. Yamazaki +2 more
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G-protein and Rho GTPase signaling in endothelial barrier regulation [PDF]
, 2019 Endothelial cells (ECs) line all blood vessels and dynamically regulate the endothelial barrier function, which is critical for proper functioning of the vasculature and human health.
Reinhard, N.R.
core
Receptor-G Protein Interaction Studied by Bioluminescence Resonance Energy Transfer: Lessons from Protease-Activated Receptor 1 [PDF]
, 2012 Since its development, the bioluminescence resonance energy transfer (BRET) approach has been extensively applied to study G protein-coupled receptors (GPCRs) in real-time and in live cells.
Abdulrahman eAl-Senaidy +5 more
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G12/13 and Gq mediate S1P2-induced inhibition of Rac and migration in vascular smooth muscle in a manner dependent on Rho but not Rho kinase [PDF]
, 2008 金沢大学医薬保健研究域医学系Aims: The lysophospholipid mediator sphingosine-1-phosphate (S1P) activates G protein-coupled receptors (GPCRs) to induce potent inhibition of platelet-derived growth factor (PDGF)-induced Rac activation and, thereby, chemotaxis in rat ...
Kaneko Shuichi +8 more
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