Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts
Life, 2021 GGDEF-containing proteins respond to different environmental cues to finely modulate cyclic diguanylate (c-di-GMP) levels in time and space, making the allosteric control a distinctive trait of the corresponding proteins.
Federico Mantoni +10 more
doaj +1 more source
Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch [PDF]
, 2017 [FeFe]-hydrogenases catalyse the reduction of protons to hydrogen at a complex 2Fe[4Fe4S] center called H-cluster. The assembly of this active site is a multistep process involving three proteins, HydE, HydF and HydG.
Acquasaliente, Laura +8 more
core +1 more source
This Is the End: Regulation of Rab7 Nucleotide Binding in Endolysosomal Trafficking and Autophagy
Frontiers in Cell and Developmental Biology, 2018 Rab7 – or in yeast, Ypt7p – governs membrane trafficking in the late endocytic and autophagic pathways. Rab7 also regulates mitochondrion-lysosome contacts, the sites of mitochondrial fission.
Christopher Stroupe
doaj +1 more source
Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif [PDF]
, 2017 Heterotrimeric G proteins are quintessential signalling switches activated by nucleotide exchange on Gα. Although activation is predominantly carried out by G-protein-coupled receptors (GPCRs), non-receptor guanine-nucleotide exchange factors (GEFs) have
Baillie, George S. +14 more
core +1 more source
A model of membrane contraction predicting initiation and completion of bacterial cell division [PDF]
, 2013 Bacterial cell division involves a complex and dynamic sequence of events whereby polymers of the protein FtsZ assemble at the division plane and rearrange to achieve the goal of contracting the cell membrane at the site of cell division, thus dividing ...
Alison Rodger +59 more
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Structural Insights into How Yrb2p Accelerates the Assembly of the Xpo1p Nuclear Export Complex
Cell Reports, 2014 Proteins and ribonucleoproteins containing a nuclear export signal (NES) assemble with the exportin Xpo1p (yeast CRM1) and Gsp1p-GTP (yeast Ran-GTP) in the nucleus and exit through the nuclear pore complex.
Masako Koyama +2 more
doaj +1 more source
Thermodynamic bounds on the ultra- and infra-affinity of Hsp70 for its substrates [PDF]
, 2017 The 70 kDa Heat Shock Proteins Hsp70 have several essential functions in living systems, such as protecting cells against protein aggregation, assisting protein folding, remodeling protein complexes and driving the translocation into organelles.
Hartich, David +3 more
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Signal Recognition Particle (SRP) and SRP Receptor: A New Paradigm for Multistate Regulatory GTPases [PDF]
, 2009 The GTP-binding proteins or GTPases comprise a superfamily of proteins that provide molecular switches in numerous cellular processes. The “GTPase switch” paradigm, in which a GTPase acts as a bimodal switch that is turned “on” and “off” by external ...
Schmid, Sandra L. +2 more
core +2 more sources
In silico docking of forchlorfenuron (FCF) to septins suggests that FCF interferes with GTP binding.
PLoS ONE, 2014 Septins are GTP-binding proteins that form cytoskeleton-like filaments, which are essential for many functions in eukaryotic organisms. Small molecule compounds that disrupt septin filament assembly are valuable tools for dissecting septin functions with
Dimitrios Angelis +3 more
doaj +1 more source
Sensor potency of the moonlighting enzyme-decorated cytoskeleton [PDF]
, 2013 Background: There is extensive evidence for the interaction of metabolic enzymes with the eukaryotic cytoskeleton. The significance of these interactions is far from clear.
Amar Patrick +5 more
core +4 more sources