Results 71 to 80 of about 84,425 (312)

Structural and biochemical characterization of the pleckstrin homology domain of the RhoGEF P-Rex2 and its regulation by PIP3

Journal of Structural Biology: X, 2019
P-Rex family Rho guanine-nucleotide exchange factors are important regulators of cell motility through their activation of a subset of small GTPases. Both P-Rex1 and P-Rex2 have also been implicated in the progression of certain cancers, including breast
Jennifer N. Cash, Prateek V. Sharma, John J.G. Tesmer   +2 more
doaj   +1 more source

Real‐Time Visualization of Isoform‐Specific RAF‐KRAS Interactions in Living Cells Using FRET‐BRET Hybrid Biosensors

Advanced Science, EarlyView.
Hybrid FRET–BRET biosensors were developed to monitor RAF–KRAS interactions in living cells. These sensors enable real‐time visualization of interaction dynamics, quantitative spectral analysis, and validation of mutation‐selective inhibitors, providing a versatile platform for probing KRAS regulation and therapeutic responses. ABSTRACT The RAS–RAF–MEK–
Jeong‐Min Go, Dahee Lee, Minji Kim, Kiseok Han, Gyuho Choi, ChanHui Song, Sanghyun Ahn, Yerim Lee, Jinyoung Lee, Yingxiao Wang, Jung‐Soo Suh, Hwayoung Yun, Tae‐Jin Kim   +12 more
wiley   +1 more source

A non-linear system patterns Rab5 GTPase on the membrane

eLife, 2020
Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors ...
Alice Cezanne, Janelle Lauer, Anastasia Solomatina, Ivo F Sbalzarini, Marino Zerial   +4 more
doaj   +1 more source

Controlling platinum, ruthenium, and osmium reactivity for anticancer drug design [PDF]

, 2009
The main task of the medicinal chemist is to design molecules that interact specifically with derailed or degenerating processes in a diseased organism, translating the available knowledge of pathobiochemical and physiological data into chemically ...
Bruijnincx, Pieter C. A., Sadler, P. J.
core   +1 more source

Single‐Cell Transcriptomics Reveals FLS2‐Dependent Hypoxia Signaling and ERF13‐Mediated Transcription During flg22‐Triggered Immunity

Advanced Science, EarlyView.
This study employs sc‐RNA sequencing, genetics, and phenotyping to systematically map the cell‐type‐specific immune responses triggered by flg22. It reveals FLS2‐dependent transcriptional reprogramming in epidermal and mesophyll cells, and uncovers crosstalk between immune and hypoxia signaling pathways.
Yaping Zhou, Aizhi Qin, Mengfan Li, Qianli Zhao, Luyao Kong, Lulu Yan, Chunyang Li, Hao Liu, Yinpeng Zhang, Jiani Long, Mengyu Liao, Mengmeng Zhou, Xiaoli Fan, Baozhen Wang, Wenxuan Kang, Shui Wang, Zhixin Liu, Xuwu Sun   +17 more
wiley   +1 more source

Identification of a guanine nucleotide exchange factor for Arf3, the yeast orthologue of mammalian Arf6.

PLoS ONE, 2007
Small G proteins of the Arf and Rab families are fundamental to the organisation and activity of intracellular membranes. One of the most well characterised of these G proteins is mammalian Arf6, a protein that participates in many cellular processes ...
Alison K Gillingham, Sean Munro
doaj   +1 more source

ITAM signaling by Vav family Rho guanine nucleotide exchange factors regulates interstitial transit rates of neutrophils in vivo. [PDF]

PLoS ONE, 2009
In response to infection, neutrophils are quickly recruited from the blood into inflamed tissues. The interstitial migration of neutrophils is crucial for the efficient capture and control of rapidly proliferating microbes before microbial growth can ...
Daniel B Graham, Bernd H Zinselmeyer, Francesca Mascarenhas, Ryan Delgado, Mark J Miller, Wojciech Swat   +5 more
doaj   +1 more source

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]

, 2013
DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Klostermeier, Dagmar, Linden, Martin H., Rudolph, Markus G., Steimer, Lenz, Wurm, Jan Philip, Wöhnert, Jens   +5 more
core   +1 more source

Extreme Kinetic Stability and RNase Resistance of Human Telomerase RNA G‐Quadruplexes Overcome by DHX36 Helicase

Advanced Science, EarlyView.
The RNA G‐quadruplexes formed at the 5'‐end of the RNA component of human telomerase possess extreme slow unfolding rate, rendering them resistant to nuclease degradation. The helicase DHX36 overcame this kinetic barrier and significantly reduced the folded fraction.
Qun Luo, Yashuo Zhang, Yingxian Qian, Huiying Zhan, Wenqiang Wu, Huijuan You   +5 more
wiley   +1 more source

Identifizierung und Charakterisierung eines neuen Bindeproteins für zyklische Nukleotide [PDF]

, 2007
Cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate are important intracellular messengers. Binding of cyclic nucleotides controls the activity of protein kinases, ion channels and guanine-nucleotide-exchange factors in many cells ...
Debowski, Katharina
core