Results 91 to 100 of about 4,450,920 (398)

CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]

, 2020
The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I, Jacinto, Filipe, Link, Wolfgang   +2 more
core   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Learning to quantify uncertainty in off-target activity for CRISPR guide RNAs [PDF]

, 2023
Furkan Özden, Péter Mináry
openalex   +1 more source

Statistical mechanics of secondary structures formed by random RNA sequences

, 2001
The formation of secondary structures by a random RNA sequence is studied as a model system for the sequence-structure problem omnipresent in biopolymers. Several toy energy models are introduced to allow detailed analytical and numerical studies. First,
A. K. Hartmann, A. Pagnani, A. Pagnani, B. Maier, C. Flamm, D. A. Huse, E. I. Shakhnovich, H. Isambert, I. L. Hofacker, I. Tinoco, Jr., J. L. Cardy, J. N. Onuchic, J. S. McCaskill, K. A. Dill, L.-H. Tang, M. Mitas, M. Zuker, P. G. de Gennes, P. G. Higgs, P. G. Higgs, R. Arratia, R. Bundschuh, R. Bundschuh, S. E. Korshunov, S. F. Edwards, S. J. Chen, S. M. Freier, S. R. Morgan, S. R. Morgan, T. C. Lubensky, T. Garel, T. Hwa, T. Hwa   +32 more
core   +2 more sources

siRNA carrying an (E)-vinylphosphonate moiety at the 5' end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2 [PDF]

, 2016
Efficient gene silencing by RNA interference (RNAi) in vivo requires the recognition and binding of the 5'- phosphate of the guide strand of an siRNA by the Argonaute protein.
Brown, C. R., Elkayam, E., Joshua-Tor, L., Manoharan, M., Parmar, R., Theile, C. S., Willoughby, J. L.   +6 more
core   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

The Escherichia coli transcriptome mostly consists of independently regulated modules [PDF]

, 2019
Underlying cellular responses is a transcriptional regulatory network (TRN) that modulates gene expression. A useful description of the TRN would decompose the transcriptome into targeted effects of individual transcriptional regulators.
A Anand, A Biton, A Delorme, A Frigyesi, A Hyvärinen, A Santos-Zavaleta, A-M Martoglio, AE Teschendorff, B Dalrymple, B Langmead, B-K Cho, B-K Cho, BM Bolstad, C Vijayendran, CL Turnbough Jr, D Kim, D Marbach, D Risso, D-S Huang, DS Latchman, E Nudler, EJ O’Brien, ENCODE Project Consortium., ER Gansner, F Pedregosa, GI Guzmán, GI Guzmán, H Zou, HS Rhee, I Kristoficova, IM Keseler, J Pouyssegur, J Utrilla, JE Galagan, JJ Faith, JM Buescher, JM Engreitz, JM Monk, JT Leek, K Valgepea, K-K Yan, KF Jensen, KJ Karczewski, L Wang, M Ester, M Kim, M Lawrence, M Moretto, M Scott, M Scott, MB Gerstein, MI Love, NE Lewis, O Alter, P Chiappetta, P Comon, PR Subbarayan, PV Phaneuf, R De Smet, R Kolter, RA LaCroix, RB D’agostino, S Gama-Castro, S Lin, SJ Larsen, SW Seo, T Baba, T Barrett, TM Henkin, W Kong, W Liebermeister, W Saelens, X Zhang, Xin Fang, XW Zhang, Y Gao, Y Yamanaka, Z Wang   +77 more
core   +3 more sources

Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding [PDF]

Cell, 2019
Ribosome assembly is an efficient but complex and heterogeneous process during which ribosomal proteins assemble on the nascent rRNA during transcription. Understanding how the interplay between nascent RNA folding and protein binding determines the fate of transcripts remains a major challenge.
Olivier, Duss, Galina A, Stepanyuk, Joseph D, Puglisi, James R, Williamson   +3 more
openaire   +3 more sources

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing.

PLoS ONE, 2010
Mitochondrial mRNA editing in Trypanosoma brucei requires the specific interaction of a guide RNA with its cognate mRNA. Hundreds of gRNAs are involved in the editing process, each needing to target their specific editing domain within the target message.
Larissa Reifur, Laura E Yu, Jorge Cruz-Reyes, Michelle Vanhartesvelt, Donna J Koslowsky   +4 more
doaj   +1 more source