Results 101 to 110 of about 4,450,920 (398)

Structure and Engineering of Francisella novicida Cas9 [PDF]

, 2016
Summary The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing.
Adams, Anders, Briner, Chylinski, Cong, Cowtan, Crooks, Deltcheva, Deveau, Emsley, Evans, Feng Zhang, Fonfara, Garneau, Gasiunas, Hiroshi Nishimasu, Hisato Hirano, Hsu, Hsu, Izuho Hatada, Jiang, Jiang, Jinek, Jinek, Jonathan S. Gootenberg, Kabsch, Kim, Kleinstiver, Kleinstiver, Lin, Mali, Mika Kimura, Mojica, Nishimasu, Nishimasu, Omar O. Abudayyeh, Osamu Nureki, Patrick D. Hsu, Ran, Ryuichiro Ishitani, Sampson, Sternberg, Sung, Takanori Nakane, Takuro Horii, Woo, Zetsche, Zuris   +47 more
core   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

Nature Communications, 2016
Genome editing in plants typically requires the expression of Cas9 and guide RNA from stably transformed plasmid DNA. Here, the authors show that successful editing can be achieved after delivery of the Cas9-guide RNA complex as a ribonucleoprotein to ...
Sergei Svitashev, Christine Schwartz, Brian Lenderts, Joshua K. Young, A. Mark Cigan   +4 more
doaj   +1 more source

Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases

Genome Biology, 2017
High-fidelity SpCas9 variants (eSpCas9 and SpCas9-HF1) have been engineered to reduce off-target effects. We found that changes in guide RNA length induced significant reductions in the editing activities of SpCas9 variants in plant cells.
Dingbo Zhang, Huawei Zhang, Tingdong Li, Kunling Chen, J. Qiu, Caixia Gao   +5 more
semanticscholar   +1 more source

Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control

ChemBioChem, 2020
Conditional control of CRISPR/Cas9 has been developed by using a variety of different approaches, many focusing on manipulation of the Cas9 protein itself.
Wes Brown, Wenyuan Zhou, A. Deiters
semanticscholar   +1 more source

In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. [PDF]

, 2019
We present in vivo sequence-specific RNA base editing via adenosine deaminases acting on RNA (ADAR) enzymes with associated ADAR guide RNAs (adRNAs). To achieve this, we systematically engineered adRNAs to harness ADARs, and comprehensively evaluated the
Chen, Genghao, Ganesh, Ashwin, Katrekar, Dhruva, Mali, Prashant, Meluzzi, Dario, Shih, Yu-Ru, Varghese, Shyni, Worlikar, Atharv   +7 more
core   +1 more source

Design principles for riboswitch function [PDF]

, 2009
Scientific and technological advances that enable the tuning of integrated regulatory components to match network and system requirements are critical to reliably control the function of biological systems. RNA provides a promising building block for the
A Belle, A Levchenko, A Zaslaver, Adam P. Arkin, B Suess, B Suess, CA Voigt, Chase L. Beisel, Christina D. Smolke, CI An, CL Beisel, DH Mathews, DJ Klein, DM Crothers, DW Selinger, E Dekel, FJ Isaacs, FJ Isaacs, GJ Leclerc, GM Emilsson, GM Suel, GR Zimmermann, HM Al-Hashimi, J Tomsic, JA Bernstein, JA Collins, JE Barrick, JF Lemay, JK Wickiser, JK Wickiser, K Lang, LJ Su, LV Danilova, M Acar, M Parisien, MN Win, MN Win, MR Bennett, MT Cheah, MT Woodside, N Hao, O Kensch, P Corish, PP Zarrinkar, PT Li, R Narsai, R Rieder, RD Jenison, RR Breaker, S Topp, S Topp, SA Lynch, SE Osborne, SK Desai, T Pan, TH Lee, TS Bayer, WC Winkler, WC Winkler, WJ Greenleaf, X Zhang, X Zhuang, Y Yokobayashi   +62 more
core   +4 more sources

RNA-Guided Human Genome Engineering via Cas9 [PDF]

Science, 2013
Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by
Mali, Prashant, Yang, Luhan, Esvelt, Kevin M., Aach, John, Güell Cargol, Marc, 1982-, DiCarlo, James E., Norville, Julie E., Church, George M.   +7 more
openaire   +3 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Internal guide RNA interactions interfere with Cas9-mediated cleavage

Nature Communications, 2016
While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.
Summer B. Thyme, Laila Akhmetova, Tessa G. Montague, Eivind Valen, Alexander F. Schier   +4 more
doaj   +1 more source