Results 11 to 20 of about 4,242,968 (247)

DeepCRISPR: optimized CRISPR guide RNA design by deep learning

Genome Biology, 2018
A major challenge for effective application of CRISPR systems is to accurately predict the single guide RNA (sgRNA) on-target knockout efficacy and off-target profile, which would facilitate the optimized design of sgRNAs with high sensitivity and ...
Guohui Chuai, Hanhui Ma, Jifang Yan, Ming Chen, Nanfang Hong, Dongyu Xue, Chi Zhou, Chenyu Zhu, Ke Chen, Bin Duan, Feng Gu, Sheng Qu, Deshuang Huang, Jia Wei, Qi Liu   +14 more
doaj   +2 more sources

A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana.

PLoS Genetics, 2020
Plant RNA viruses are used as delivery vectors for their high level of accumulation and efficient spread during virus multiplication and movement. Utilizing this concept, several viral-based guide RNA delivery platforms for CRISPR-Cas9 genome editing ...
Basudev Ghoshal, Brandon Vong, Colette L Picard, Suhua Feng, Janet M Tam, Steven E Jacobsen   +5 more
doaj   +2 more sources

A population study of the minicircles in Trypanosoma cruzi: predicting guide RNAs in the absence of empirical RNA editing [PDF]

BMC Genomics, 2007
Background The structurally complex network of minicircles and maxicircles comprising the mitochondrial DNA of kinetoplastids mirrors the complexity of the RNA editing process that is required for faithful expression of encrypted maxicircle genes ...
Martinez, LL Isadora Trejo, Sturm, Nancy R, Thomas, Sean, Westenberger, Scott J   +3 more
core   +5 more sources

Multi-locus CRISPRi targeting with a single truncated guide RNA. [PDF]

Nat Commun
A critical goal in functional genomics is evaluating which non-coding elements contribute to gene expression, cellular function, and disease. Functional characterization remains a challenge due to the abundance and complexity of candidate elements. Here,
Moore MM, Wekhande S, Issner R, Collins A, Cruz AJ, Liu YV, Javed N, Casaní-Galdón S, Buenrostro JD, Epstein CB, Mattei E, Doench JG, Bernstein BE, Shoresh N, Najm FJ.   +14 more
europepmc   +2 more sources

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

Cell, 2014
Hiroshi Nishimasu, F. Ann Ran, Patrick D. Hsu, Silvana Konermann, Soraya I. Shehata, Naoshi Dohmae, Ryuichiro Ishitani, Feng Zhang, Osamu Nureki   +8 more
openalex   +2 more sources

PlmCas12e (CasX2) cleavage of CCR5: impact of guide RNA spacer length and PAM sequence on cleavage activity

RNA Biology, 2023
Gene editing using CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR-associated) is under development as a therapeutic tool for the modification of genes in eukaryotic cells.
David A. Armstrong, Taylor R. Hudson, Christine A. Hodge, Thomas H. Hampton, Alexandra L. Howell, Matthew S. Hayden   +5 more
doaj   +1 more source

Molecular dynamics simulations of chemically modified ribonucleotides [PDF]

In: Barciszewski, J. (eds) RNA Structure and Function. RNA Technologies, vol 14 (2023). Springer, Cham, 2022
Post-transcriptional modifications are crucial for RNA function, with roles ranging from the stabilization of functional RNA structures to modulation of RNA--protein interactions. Additionally, artificially modified RNAs have been suggested as optimal oligonucleotides for therapeutic purposes. The impact of chemical modifications on secondary structure
arxiv   +1 more source

Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3

Proceedings of the National Academy of Sciences of the United States of America, 2021
Significance Genome editing technologies enable the permanent repair of disease-causing genetic mutations. However, the application of this technology has been limited by the technical challenge of achieving safe, effective, and specific in vivo delivery
Min Qiu, Z. Glass, Jinjin Chen, Mary E. Haas, Xin Jin, Xuewei Zhao, Xuehui Rui, Zhongfeng Ye, Yamin Li, Feng Zhang, Qiaobing Xu   +10 more
semanticscholar   +1 more source

Crosstalk between CRISPR-Cas9 and the human transcriptome

Nature Communications, 2022
The off-target effects of CRISPR-Cas9 are thought to be mediated by its cognate guide RNA. Here the authors show that Cas9 independently interacts with the human transcriptome, correlating with elevated RNA editing even under guide RNA co-expression.
Aaron A. Smargon, Assael A. Madrigal, Brian A. Yee, Kevin D. Dong, Jasmine R. Mueller, Gene W. Yeo   +5 more
doaj   +1 more source

Characterization of a Programmable Argonaute Nuclease from the Mesophilic Bacterium Rummeliibacillus suwonensis

Biomolecules, 2022
Prokaryotic Argonautes (pAgos) from mesophilic bacteria are attracting increasing attention for their genome editing potential. So far, it has been reported that KmAgo from Kurthia massiliensis can utilize DNA and RNA guide of any sequence to effectively
Xiaoman Jiang, Yang Liu, Qi Liu, Lixin Ma   +3 more
doaj   +1 more source