Results 21 to 30 of about 4,450,920 (398)
Nucleic Acids Research, 1997 The RNA editing process in protozoan parasites is controlled by small RNA molecules known as guide RNAs (gRNAs). The gRNA database is a comprehensive compilation of published guide RNA sequences from eight different kinetoplastid organisms. In addition to the RNA primary sequences, information on the gene localization, the experimental verification of ...
A E, Souza, T, Hermann, H U, Göringer
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Nucleic Acids Research, 1998 Guide RNAs (gRNAs) are small, metabolically stable RNA molecules which perform a pivotal, template-like function during the RNA editing process in kinetoplastid protozoa. The gRNA database currently contains 250 guide RNA sequences as well as secondary and tertiary structure models and other relevant information.
A E, Souza, H U, Göringer
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Cas9 deactivation with photocleavable guide RNAs [PDF]
Molecular Cell, 2021 Precise control of CRISPR-Cas9 would improve its safety and applicability. Controlled CRISPR inhibition is a promising approach but is complicated by separate inhibitor delivery, incomplete deactivation, and slow kinetics. To overcome these obstacles, we engineered photocleavable guide RNAs (pcRNAs) that endow Cas9 nucleases and base editors with a ...
Roger S, Zou +3 more
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CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris. [PDF]
Synth Syst BiotechnolChen K +9 more
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Genome Biology, 2016 BackgroundThe success of the CRISPR/Cas9 genome editing technique depends on the choice of the guide RNA sequence, which is facilitated by various websites.
M. Haeussler +11 more
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CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion
BMC Genomics, 2022 Background Recent discovery of the gene editing system - CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) associated proteins (Cas), has resulted in its widespread use for improved understanding of a variety of biological systems ...
Alexander Krohannon +5 more
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Structural insights into RNA processing by the human RISC-loading complex. [PDF]
, 2009 Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC).
AD Haase +41 more
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The guide RNA database (3.0) [PDF]
Nucleic Acids Research, 1999 The RNA editing process within the mitochondria of kinetoplastid organisms is controlled by small, trans -acting RNA molecules referred to as guide RNAs. The guide RNA database is a compilation of published guide RNA sequences, currently containing 254 entries from 11 different organisms.
S, Hinz, H U, Göringer
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CRISPR guides induce gene silencing in plants in the absence of Cas
Genome Biology, 2022 Background RNA-targeting CRISPR-Cas can provide potential advantages over DNA editing, such as avoiding pleiotropic effects of genome editing, providing precise spatiotemporal regulation, and expanded function including antiviral immunity.
Veerendra Kumar Sharma +7 more
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Traffic into silence: endomembranes and post-transcriptional RNA silencing. [PDF]
, 2014 microRNAs (miRNAs) and small interfering RNAs (siRNAs) are small RNAs that repress gene expression at the post-transcriptional level in plants and animals. Small RNAs guide Argonaute-containing RNA-induced silencing complexes to target RNAs in a sequence-
Chen, Xuemei +2 more
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