Results 121 to 130 of about 3,793,883 (300)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

A Guideline for Guidelines: A Novel Method to Assess the Helpfulness of Medical Guidelines

Journal of Clinical Medicine
Background/Objectives: The recommendations included in medical guidelines (GLs) provide important help to medical professionals for making clinical decisions regarding the diagnosis and treatment of various diseases. However, there are no systematic methods to measure the helpfulness of GLs.
Akos Koller, Johanna Takács
openaire   +2 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Effect of preterm birth on neonatal, infant, child, young adult and adult mortality: a systematic review and meta-analysis

Journal of Perinatal Medicine
Preterm birth is associated with a higher risk of mortality. In 2020, the global incidence of preterm births was reported at 9.9 per 100 live births. This meta-analysis was undertaken to evaluate the effect of preterm birth on mortality among various age
Malshe Nandini Shashikant, Kapoor Anuva, Deshmukh Kalyani P., Goyal Chanchal, Gandhi Aravind P.   +4 more
doaj   +1 more source

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

Improving the quality of WHO guidelines over the last decade: progress and challenges

The Lancet Global Health, 2017
Susan L Norris, Nathan Ford
doaj   +1 more source

Research progress and implications of the application of large language model in shared decision-making in China’s healthcare field

Frontiers in Public Health
Shared Decision Making (SDM), as a modern medical decision-making model emphasizing patient participation, faces multidimensional challenges in China, including uneven distribution of medical resources, knowledge gaps, and inadequate cultural adaptation.
Xuejing Li, Xuejing Li, Xuejing Li, Sihan Chen, Sihan Chen, Sihan Chen, Meiqi Meng, Meiqi Meng, Meiqi Meng, Ziyan Wang, Ziyan Wang, Ziyan Wang, Hongzhan Jiang, Hongzhan Jiang, Hongzhan Jiang, Yufang Hao, Yufang Hao, Yufang Hao   +17 more
doaj   +1 more source

Guidelines for the Use of Guidelines [PDF]

Prehospital and Disaster Medicine, 1993
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Cholera Outbreak Guidelines: Preparedness, prevention and control [PDF]

, 2012
This practical field guide brings together lessons learned from Oxfam's past interventions in the prevention and control of cholera, and other related guidance.
Lamond, Elizabeth, Kinyanjui, Jesee
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