Results 31 to 40 of about 3,013 (178)

GUV preparation and imaging: Minimizing artifacts

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2010
The components of biological membranes are present in a physical mixture. The nonrandom ways that the molecules of lipids and proteins mix together can strongly influence the association of proteins with each other, and the chemical reactions that occur in the membrane, or that are mediated by the membrane.
Morales-Penningston, Nelson F., Wu, Jing, Farkas, Elaine R., Goh, Shih Lin, Konyakhina, Tatyana M., Zheng, Judy Y., Webb, Watt W., Feigenson, Gerald W.   +7 more
openaire   +2 more sources

Volatile Relay Biosensing and Mobile Phone Imaging for Detection of Influenza Virus

Advanced Functional Materials, EarlyView.
Detection of volatile organic compounds is performed via a coupled enzymatic assay with mobile phone detection of the signal. Specific application demonstrated herein is the detection of influenza virus, across multiple levels of biological complexity, up to patient‐derived samples.
Dante G. Andersen, Josefine H. Jakobsen, Mikkel Høj Nielsen, Shreyans Chatterjee, Detlef Michel, Jan Münch, Rüdiger Gross, Alexander N. Zelikin   +7 more
wiley   +1 more source

Semisynthesis of functional transmembrane proteins in GUVs [PDF]

, 2021
Abstract Cellular transmembrane (TM) proteins are essential sentries of the cell facilitating cell-cell communication, internal signaling, and solute transport. Reconstituting functional TM proteins into model membranes remains a challenge due to the difficulty of expressing hydrophobic TM domains and the required use of detergents ...
K. A. Podolsky, T. Masubuchi, G. T. Debelouchina, E. Hui, N. K. Devaraj   +4 more
openaire   +1 more source

Self‐Assembled Lipids as Soft Dielectrics

Advanced Functional Materials, EarlyView.
Self‐assembled lipid bilayers serve as soft dielectric materials for ultrathin electronics. Using liquid‐metal parallel‐plate junctions, the capacitance and dielectric breakdown of dehydrated lipid films are characterized. Dielectric behavior is controlled by lipid molecular structure, cholesterol content, and assembly conditions, providing molecular ...
Hao Yang, Yiding Li, Jitong Ren, Yu Shi, Charles M. Schroeder   +4 more
wiley   +1 more source

Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology

Gels
Giant unilamellar vesicles (GUVs) are versatile cell models in biomedical and environmental research. Of the various GUV production methods, hydrogel-assisted GUV production is most easily implemented in a typical biological laboratory. To date, agarose,
Cherng-Wen Darren Tan, Magdalena Schöller, Eva-Kathrin Ehmoser   +2 more
doaj   +1 more source

Programming Nanoscale Reaction Environments via Peptide Localization for Gold Nanoparticle Growth in Liposomal Nanoreactors

Advanced Functional Materials, EarlyView.
The liposome nanoreactor method enables uniform metal nanoparticle synthesis using the interior as a reaction environment. We demonstrate that peptide sequences and lipid compositions act as a functional molecular switch to control reaction environments in liposome nanoreactors.
Yuya Abe, Joseph Fox, Zabeada Aslam, Kevin Critchley, Stephen D. Evans, Mina Okochi, Masayoshi Tanaka   +6 more
wiley   +1 more source

Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos

Scientific Reports, 2022
Giant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes ...
Luana Teixeira Rodrigues Rossi, Giovana Barros Nunes, Cíntia Rodrigues da Silva, Hugo de Rossi, Priscila Helena dos Santos, Marcelo Fábio Gouveia Nogueira, Pedro Henrique Benites Aoki, Gisele Zoccal Mingoti   +7 more
doaj   +1 more source

Azobenzene's Cross‐Scale Optics and Photonics: Molecular Photoswitching, Mesoscopic Material Motions, and Adaptive Devices

Advanced Materials, EarlyView.
Azobenzene photoswitches translate molecular‐scale E/Z photoisomerization into macroscopic material responses and device‐level photonic functions. This Review highlights how azobenzene research has evolved from molecular photochemistry to photoalignment, mass migration, photomechanics, and heat release, ultimately enabling holography, reconfigurable ...
Heeju Son, Soyul Kwak, Heerin Noh, Minsang Kim, Doyeon Noh, Subhayan Chakraborty, Jaewon Lee, YongDeok Cho, Kyeongsoo Kim, Taewoong Eom, Moonyoung Kim, Jangwon Kim, Hoyoung Lee, Soyoung Lee, Jaehun Kim, Grace G. D. Han, Shin‐Tson Wu, Stefano Oscurato, Arri Priimagi, Marina Saphiannikova, Seungwoo Lee   +20 more
wiley   +1 more source

β‐Ketonitrile‐Modified Prodan Derivatives as Large‐Stokes‐Shift Solvatochromic Fluorophores With Enhanced Brightness and Ratiometric Thermometric Response

Chemistry – A European Journal, EarlyView.
We report a systematic study of β‐ketonitrile–modified Prodan‐type solvatochromic fluorophores. The β‐ketonitrile substitution induces marked bathochromic shifts in absorption and emission and significantly enhances fluorescence quantum yields in nonpolar solvents.
Hiroaki Asakawa, Takuya Tanaka, Kensei Konishi, Gen‐ichi Konishi   +3 more
wiley   +1 more source

RETROSPECTIVE ANALYSIS OF RABIES EPIZOOTIC SITUATION IN MOSCOW REGION IN 2010-2016

Ветеринария сегодня, 2018
Rabies is a viral disease of animals and humans that is characterized by signs of polioencephalomyelitis and is in most cases lethal. This is one of the oldest and most dangerous infectious diseases.
A. V. Paroshin, S. V. Voskresensky, A. A. Mukovnin, A. V. Yenshin, A. Ye. Metlin, K. N. Gruzdev   +5 more
doaj