Results 61 to 70 of about 4,029 (204)

Validation of a real-time PCR forHaemophilus parasuis [PDF]

Journal of Applied Microbiology, 2010
To validate a real-time PCR test for the diagnosis of Glässer's disease, a major pig disease caused by Haemophilus parasuis.The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples of DNA from tissues and fluids of 16 experimentally challenged
Turni, C., Pyke, M., Blackall, P.J.
openaire   +5 more sources

Isolation of Haemophilus parasuis from Diagnostic Samples in the South of Brazil

Brazilian Journal of Veterinary Pathology, 2011
Haemophilus parasuis is one of the first and most prevalent colonizers of piglets, affecting the swine population from 2 weeks to 4 months of age. In this work, the relative incidence of H.
Mario L. Teixeira, Suzana S. Kuchiishi, Adriano Brandelli   +2 more
doaj   +1 more source

Identification of a novel Haemophilus parasuis-specific B cell epitope using monoclonal antibody against the OppA protein. [PDF]

PLoS ONE, 2014
Monoclonal antibody (MAb) 1B3 against Haemophilus parasuis (H. parasuis) was generated by fusing SP2/0 murine myeloma cells and spleen cells from BALB/c mice immunized with the whole-bacterial-cell suspension of H. parasuis HS80 (serotype 5). The MAb 1B3
Nan Zheng, Zheng Chai, Fang Fu, Fucheng Jiang, Xiangling Wang, Xueyun Zhang, Zhuo Wang, Xi Li   +7 more
doaj   +1 more source

Differences in phagocytosis susceptibility inHaemophilus parasuisstrains [PDF]

Veterinary Research, 2009
Haemophilus parasuis is a colonizer of the upper respiratory tract of healthy pigs, but virulent strains can cause a systemic infection characterized by fibrinous polyserositis, commonly known as Glässer's disease. The variability in virulence that is observed among H.
Olvera, Alexandre, Ballester, Maria, Nofrarías, Miquel, Sibila, Marina, Aragon, Virginia   +4 more
openaire   +4 more sources

The Oral Tolerance as a Complex Network Phenomenon [PDF]

, 2015
Date of Acceptance: 23/05/2015 Funding: MSB acknowledges the Engineering and Physical Sciences Research Council (EPSRC) - UK grant EP/I032606/1. PJM and MD received regular scholarships from the Brazilian the following agency: Higher Education Personnel ...
Baptista, Murilo Da Silva, Delgobo, Murilo, Marino, Giovani Favero, Miranda, Pedro Jeferson, Paludo, Katia Sabrina, Pinto, Sandro Ely De Soula   +5 more
core   +10 more sources

Padronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos. [PDF]

, 2016
Based on diagnostic tests for the detection of nucleic acids without amplification through the use of gold nanoparticles (AuNPs) have been described for various diseases.
BRANDÃO, L. N. S., CHITARRA, C. S., DUTRA, V., KLEIN, C. S., MARUYAMA, F. H., NAKAZATO, L., PITCHENIM, L. C., SILVA, G. F. R. da   +7 more
core   +1 more source

Pharmacokinetic/pharmacodynamic evaluation of marbofloxacin in the treatment of Haemophilus parasuis and Actinobacillus pleuropneumoniae infections in nursery and fattener pigs using Monte Carlo simulations. [PDF]

, 2014
This study evaluated the theoretical clinical outcome of three marbofloxacin posology regimens in two groups of pigs (weaners and fatteners) for the treatment of Actinobacillus pleuropneumoniae (App) and Haemophilus parasuis (Hp) infection and the ...
El Garch, Farid, Fraile Sauce, Lorenzo José, Giboin, Henry, Schneider, M., Vilalta, Carles   +4 more
core   +2 more sources

Application of ERIC PCR for the comparison of isolates of Haemophilus parasuis [PDF]

Australian Veterinary Journal, 2000
ObjectiveTo validate a polymerase chain reaction (PCR) based method, Enterobacterial Repetitive Intergenic Consensus‐PCR (ERIC‐PCR), for the fingerprinting ofHaemophilus parasuisstrains and to use that method to differentiate isolates from apparently related outbreaks of Glässers disease on three pig farms.DesignERIC‐PCR was evaluated by comparing 15 ...
Rafiee, M., Bara, M., Stephens, C.P., Blackall, P.J.   +3 more
openaire   +5 more sources

Epidemiologia molecular de Haemophilus parasuis [PDF]

Ciência Rural, 2009
A maioria dos estudos epidemiológicos envolvendo Haemophilus parasuis isolados de rebanhos suínos brasileiros se baseia em sorotipagem. Entretanto, uma alta porcentagem de amostras não é sorotipável. Técnicas moleculares têm sido utilizadas com sucesso para caracterizar a diversidade dos isolados de H.
Macêdo, Núbia Resende de, Oliveira, Simone Rodrigues, Lage, Andrey Pereira, Guedes, Roberto Maurício Carvalho   +3 more
openaire   +3 more sources

Droplet Digital CRISPR for Nucleic Acid Detection

Advanced Science, Volume 13, Issue 14, 9 March 2026.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber, Ming Li   +4 more
wiley   +1 more source