Results 51 to 60 of about 611,282 (250)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Most Common Patient Hair Questions and Answers

, 2019
Patients often have a number of questions regarding changes in their hair. This chapter contains the most common questions patients ask as well as the answers to these questions.
Antonella Tosti, Griggs, Jacob, Burroway, Brandon, Brandon Burroway, Jacob Griggs, Tosti, Antonella   +5 more
core   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

FEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno, Shota Yamazaki, Hiromichi Hoshina, Masahiko Harata   +3 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

A Case Series on Minimally Invasive Upper Arm Contouring: Efficacy of Combined Renuvion Helium Plasma and Icoone Multi‐Micro Alveolar Stimulation for Fat Reduction

Clinical Case Reports
The combination of Renuvion helium plasma and Icoone Multi Micro Alveolar Stimulation is an effective, minimally invasive approach for reducing upper arm fat and improving skin texture.
Mohammad Ali Nilforoushzadeh, Alireza Jafarzadeh, Tannaz Fakhim, Masoumeh Mohamadi, Fereshteh Salarvand, Farnaz Parvaresh, Niloufar Najar Nobari   +6 more
doaj   +1 more source

Implementation of the synthetic arabinose 5-phosphate dependent glycolaldehyde assimilation (SAGA) pathway in Pseudomonas putida

Frontiers in Bioengineering and Biotechnology
Ethylene glycol (EG) is a promising substrate for biochemical product syntheses as it can be sourced sustainably from CO2 or plastic waste. The synthetic, arabinose 5-phosphate dependent glycolaldehyde assimilation (SAGA) pathway has been recently ...
Alrik Titze, Nils Wagner, Cláudio J. R. Frazão, Thomas Walther   +3 more
doaj   +1 more source

A Histological and Clinical Evaluation of Plasma as a Graft Holding Solution and Its Efficacy in Terms of Hair Growth and Graft Survival

Indian Journal of Plastic Surgery, 2019
Background There is a time lag between hair follicle harvesting and implantation, during this time hair follicles suffer ischemic injury. We need a holding medium or a solution to minimize or neutralize ischemic injury.
Anil Kumar Garg, Seema Garg
doaj   +1 more source