Results 181 to 190 of about 8,406 (222)
Some of the next articles are maybe not open access.
Tertiary Structure Stabilization Promotes Hairpin Ribozyme Ligation
Biochemistry, 1999The hairpin ribozyme catalyzes a reversible RNA cleavage reaction that participates in processing intermediates of viral satellite RNA replication in plants. A minimal hairpin ribozyme consists of two helix-loop-helix segments. These segments associate noncoaxially in the active folded structure in a way that brings catalytically important loop ...
openaire +2 more sources
Crystallization of the Hairpin Ribozyme: Illustrative Protocols
2004Conditions and techniques that result in successful crystallization differ from RNA to RNA. However, there are some general principles that facilitate crystallization of most RNAs. Three procedures that were instrumental in obtaining well-ordered crystals of the hairpin ribozyme are described in this chapter.
Peter B, Rupert +1 more
openaire +2 more sources
The hairpin ribozyme: from crystal structure to function
Biochemical Society Transactions, 2002The hairpin ribozyme is one of four known natural catalytic RNAs that carry out sequence-specific cleavage of RNA. It is of particular biochemical interest because, unlike ‘classic’ ribozymes, such as the group I intron, it appears not to employ metal ions as catalytic cofactors.
A R, Ferré-D'amaré, P B, Rupert
openaire +2 more sources
The catalytic mechanism of the hairpin ribozyme
Biochemical Society Transactions, 2002Evidence that hairpin ribozymes function in the absence of bivalent cation cofactors suggests that active site nucleobases might participate directly in catalytic chemistry. We have adopted an abasic ribozyme rescue strategy to begin to dissect the roles of specific nucleobases in hairpin ribozyme activity. Loss of one active site nucleobase, G8, could
openaire +2 more sources
Enhanced Folding of Hairpin Ribozymes with Replaced Domains
Biochemistry, 1996Reversely joined ribozymes (Komatsu et al., 1995) have been proven to be active. Here we describe the construction of hairpin ribozymes with separated domains, but containing complementary arms for association of the two domains. Linker nucleotides were inserted between the arms and domains. These ribozymes were active under the standard conditions (12
Y, Komatsu, I, Kanzaki, E, Ohtsuka
openaire +2 more sources
Hairpin Ribozyme-Catalyzed Ligation in Water-Alcohol Solutions
Oligonucleotides, 2005The hairpin ribozyme (HPR) is a naturally existing RNA that catalyzes site-specific RNA cleavage and ligation. At 37 degrees C and in the presence of divalent metal ions (M(2+)), the HPR efficiently cleaves RNA substrates in trans. Here, we show that the HPR can catalyze efficient M(2+)-independent ligation in trans in aqueous solutions containing any ...
Alexander V, Vlassov +2 more
openaire +2 more sources
Design and Optimization of Sequence-Specific Hairpin Ribozymes
2004The hairpin ribozyme belongs to a group of small catalytic RNAs that have been extensively used to trans-cleave RNA molecules. Many efforts have been made to elucidate its reaction mechanism, and there is great interest in designing hairpin ribozymes with improved catalytic activity for use in the development of agents that specifically inactivate RNA ...
Cristina, Romero-López +3 more
openaire +2 more sources
De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide
Nature Nanotechnology, 2021Batsaikhan Mijiddorj +2 more
exaly
Ribozyme Chemistry: To Be or Not To Be under High Pressure
Chemical Reviews, 2020Marie-Christine Maurel, Fabrice Leclerc
exaly