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The Hairpin Ribozyme: Discovery and Development for Gene Therapy
2003The hairpin ribozyme is a member of a family of small RNA endonucleases, which includes hammerhead, human hepatitis delta virus, Neurospora VS, and the lead-dependent catalytic RNAs. All these catalytic RNAs reversibly cleave the phosphodiester bond of substrate RNA to generate 5′-hydroxyl and 2′,3′-cyclic phosphate termini.
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Kinetics of hairpin ribozyme cleavage in yeast.
RNA (New York, N.Y.), 1997Hairpin ribozymes catalyze a self-cleavage reaction that provides a simple model for quantitative analyses of intracellular mechanisms of RNA catalysis. Decay rates of chimeric mRNAs containing self-cleaving ribozymes give a direct measure of intracellular cleavage kinetics in yeast.
Donahue, Christine Patricia +1 more
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Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes
2004The application of conventional enzymological methods to the study of hairpin and hammerhead ribozymes has led to valuable insights into the mechanisms by which these small RNAs catalyze phosphodiester cleavage and ligation reactions. Here, protocols are presented for measuring rate constants for simple cleavage and ligation reactions mediated by ...
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Mechanistic studies of the hairpin ribozyme
Collection of Czechoslovak Chemical Communications, 1996Francesca Gill +2 more
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Ligation of RNA Molecules by the Hairpin Ribozyme
2003Alfredo Bertal-Herranz, John M. Burke
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Catalytic and Structural Assays for the Hairpin Ribozyme
2001Robert Pinard +2 more
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Determination of Catalytic Parameters for Hairpin Ribozymes
2003Andrew Siwkowski +2 more
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