Results 91 to 100 of about 5,757 (229)

A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification

Frontiers in Microbiology
Extremophilic proteins are valuable in various fields, but their expression can be challenging in traditional hosts like Escherichia coli due to misfolding and aggregation. Haloferax volcanii (H.
Ram Karan, Ram Karan, Dominik Renn, Thorsten Allers, Magnus Rueping, Magnus Rueping   +5 more
doaj   +1 more source

Toxicity Assessment and Bioremediation of Deep Eutectic Solvents by Haloferax mediterranei: A Step toward Sustainable Circular Chemistry

ChemSusChem, Volume 18, Issue 16, August 6, 2025.
The toxicity and biodegradability of deep eutectic solvents (DESs) can be evaluated using Haloferax mediterranei as a novel model organism. The haloarchaeon metabolizes specific DES components, highlighting its potential for DES bioremediation. These findings support the use of H.
Guillermo Martínez, Gabriela Guillena, Rosa María Martínez‐Espinosa   +2 more
wiley   +1 more source

Haloferax volcanii cells lacking the flagellin FlgA2 are hypermotile [PDF]

Microbiology, 2013
Motility driven by rotational movement of flagella allows bacteria and archaea to seek favourable conditions and escape toxic ones. However, archaeal flagella share structural similarities with bacterial type IV pili rather than bacterial flagella. The Haloferax volcanii genome contains two flagellin genes, flgA1 and flgA2.
Manuela, Tripepi, Rianne N, Esquivel, Reinhard, Wirth, Mechthild, Pohlschröder   +3 more
openaire   +2 more sources

Ultrasound Imaging Properties of Heterologously Synthesized Gas Vesicles from Halophilic Archaeon

Nanomaterials
Biosynthetic gas vesicles (GVs), as novel nanoscale ultrasound contrast agents, exhibit unique potential in biomedical ultrasound imaging. For example, they are expected to have better tissue penetration through the tumor vasculature for detecting tumor ...
Wenze Ou, Chenxing Liu, Yuanyuan Wang, Qiuxia Fu, Wei Liu, Huan Long, Fei Yan   +6 more
doaj   +1 more source

Biosynthesis and Role of N-Linked Glycosylation in Cell Surface Structures of Archaea with a Focus on Flagella and S Layers

International Journal of Microbiology, 2010
The genetics and biochemistry of the N-linked glycosylation system of Archaea have been investigated over the past 5 years using flagellins and S layers as reporter proteins in the model organisms, Methanococcus voltae, Methanococcus maripaludis, and ...
Ken F. Jarrell, Gareth M. Jones, Divya B. Nair   +2 more
doaj   +1 more source

Genetic and physical mapping of DNA replication origins in Haloferax volcanii. [PDF]

PLoS Genetics, 2007
The halophilic archaeon Haloferax volcanii has a multireplicon genome, consisting of a main chromosome, three secondary chromosomes, and a plasmid. Genes for the initiator protein Cdc6/Orc1, which are commonly located adjacent to archaeal origins of DNA ...
Cédric Norais, Michelle Hawkins, Amber L Hartman, Jonathan A Eisen, Hannu Myllykallio, Thorsten Allers   +5 more
doaj   +1 more source

Strategies for the Reconstitution and Purification of Haloarchael Protein RadA

Pakistan Journal of Analytical & Environmental Chemistry, 2019
DNA repair proteins in halophilic organisms are interesting to study in the context of understanding the dynamics of protein-DNA interaction and their adaptation to perform biochemical activities at high osmolarity. Successful expression and purification
Bushra B. Patoli, Atif A. Patoli
doaj   +1 more source

Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax volcanii

Frontiers in Microbiology, 2020
In recent years, fluorescence microscopy techniques for the localization and tracking of single molecules in living cells have become well-established and are indispensable tools for the investigation of cellular biology and in vivo biochemistry of many ...
Bartosz Turkowyd, Sandra Schreiber, Julia Wörtz, Ella Shtifman Segal, Moshe Mevarech, Iain G. Duggin, Anita Marchfelder, Ulrike Endesfelder, Ulrike Endesfelder   +8 more
doaj   +1 more source

Improving the genetic system for Halorubrum lacusprofundi to allow in-frame deletions

Frontiers in Microbiology, 2023
Halorubrum lacusprofundi is a cold-adapted halophilic archaeon isolated from Deep Lake, Antarctica. Hrr. lacusprofundi is commonly used to study adaptation to cold environments and thereby a potential source for biotechnological products.
L. Johanna Gebhard, Iain G. Duggin, Susanne Erdmann   +2 more
doaj   +1 more source

DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.

PLoS ONE, 2014
Haloferax volcanii uses extracellular DNA as a source for carbon, nitrogen, and phosphorous. However, it can also grow to a limited extend in the absence of added phosphorous, indicating that it contains an intracellular phosphate storage molecule.
Karolin Zerulla, Scott Chimileski, Daniela Näther, Uri Gophna, R Thane Papke, Jörg Soppa   +5 more
doaj   +1 more source