Results 101 to 110 of about 16,494 (236)

In vitro activity of minimised hammerhead ribozymes

Nucleic Acids Research, 1995
A number of minimised hammerhead ribozymes (minizymes) which lack stem II have been kinetically characterised. These minizymes display optimal cleavage activity at temperatures around 37 degrees C. The cleavage reactions of the minizymes are first order in hydroxide ion concentration up to around pH 9.3 above which the cleavage rate constants decline ...
P, Hendry, M J, McCall, F S, Santiago, P A, Jennings   +3 more
openaire   +3 more sources

Nonenzymatic copying of RNA templates containing all four letters is catalyzed by activated oligonucleotides

eLife, 2016
The nonenzymatic replication of RNA is a potential transitional stage between the prebiotic chemistry of nucleotide synthesis and the canonical RNA world in which RNA enzymes (ribozymes) catalyze replication of the RNA genomes of primordial cells ...
Noam Prywes, J Craig Blain, Francesca Del Frate, Jack W Szostak   +3 more
doaj   +1 more source

The model structure of the hammerhead ribozyme formed by RNAs of reciprocal chirality. [PDF]

Biosci Rep, 2021
Wyszko E, Popenda M, Gudanis D, Sarzyńska J, Belter A, Perrigue P, Skowronek P, Rolle K, Barciszewski J.   +8 more
europepmc   +1 more source

Automated design of hammerhead ribozymes and validation by targeting the PABPN1 gene transcript [PDF]

, 2015
Nawwaf Kharma, Luc Varin, Aida Abu‐Baker, Jonathan Ouellet, Sabrine Najeh, Mohammad-Reza Ehdaeivand, Gabriel Belmonte, Anas Ambri, Guy A. Rouleau, Jonathan Perreault   +9 more
openalex   +1 more source

Hammerhead Ribozyme Mediated Suppression of Chikungunya Virus Replication in Vero Cells

, 2022
Velmurugan Balaraman
openalex   +1 more source

Unexpected Anisotropy in Substrate Cleavage Rates by Asymmetric Hammerhead Ribozymes [PDF]

, 1996
Philip Hendry, Maxine J. McCall
openalex   +1 more source

The Suppression of MUC1 Expression in Cultured Cholangiocarcinoma Cells Using Antisense MUC1 Ribozyme Strategy

Siriraj Medical Journal, 2002
Ribozyme strategy has been employed to suppress the expression of target genes with a better efficacy than that of a regular antisense RNA strategy due to its favorable stoichiometry of the hybridization.
Valla Wamanutajinda, Adisak Wongkajornsilp, Yudhtana Sattawatthamrong, Sukit Huabprasert   +3 more
doaj  

Internal initiation of reverse transcription in a Penelope-like retrotransposon

Mobile DNA
Eukaryotic retroelements are generally divided into two classes: long terminal repeat (LTR) retrotransposons and non-LTR retrotransposons. A third class of eukaryotic retroelement, the Penelope-like elements (PLEs), has been well-characterized ...
Chris J. Frangieh, Max E. Wilkinson, Daniel Strebinger, Jonathan Strecker, Michelle L. Walsh, Guilhem Faure, Irina A. Yushenova, Rhiannon K. Macrae, Irina R. Arkhipova, Feng Zhang   +9 more
doaj   +1 more source

Cellular delivery of hammerhead ribozymes [PDF]

, 1997
Hammerhead ribozymes are potent RNA molecules which have the potential to specifically inhibit gene expression by catalysing the trans-cleavage of mRNAs. However, they are unstable in biological fluids and cellular delivery poses a problem. Site-specific chemical modification of hammerhead ribozymes was evaluated as a means of enhancing biological ...
openaire   +1 more source

Peptide nucleic acid (PNA) is capable of enhancing hammerhead ribozyme activity with long but not with short RNA substrates [PDF]

, 1997
Eckhard Jankowsky
openalex   +1 more source