Results 171 to 180 of about 16,494 (236)

Principles of <i>in vitro</i> selection of ribozymes from random sequence libraries. [PDF]

J R Soc Interface
Higgs PG, Muller UF.
europepmc   +1 more source

Efficient genome editing by CRISPR-Cas12a in common wheat via shoot apical meristem delivery. [PDF]

Plant Physiol
Luo W, Ors S, Fang H, Zeng M, Cheng Y, Sidawy AE, Qi Y.   +6 more
europepmc   +1 more source

Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae. [PDF]

N Biotechnol
Xi C, Chiu S, Voje WE, Carothers JM, Moon TS.   +4 more
europepmc   +1 more source

Expression of the <i>Nicotiana benthamiana</i> Retrozyme 1 (NbRZ1) Genomic Locus. [PDF]

Plants (Basel)
Lezzhov AA, Atabekova AK, Chergintsev DA, Solovyev AG, Morozov SY.   +4 more
europepmc   +1 more source

Effects of lipid membranes on RNA catalytic activity and stability. [PDF]

Biol Cell
Czerniak T, Saenz JP.
europepmc   +1 more source

Exonuclease-fused CRISPR-cas system enhances targeted genome editing for functional genomics in soybean. [PDF]

BMC Plant Biol
Devkar V, Ghose K, D'Agostino L, Patil GB.   +3 more
europepmc   +1 more source

Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system. [PDF]

Sci Rep
Moroi K, Yamamoto T, Kurita T.
europepmc   +1 more source

Plasmid-Based Reverse Genetics System Enabling One-Step Generation of Genotype 3 Hepatitis E Virus. [PDF]

Viruses
Kobayashi T, Nishiyama T, Yamada K, Murata K, Okamoto H.   +4 more
europepmc   +1 more source

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Highly active low magnesium hammerhead ribozyme.

The Journal of Biochemistry, 2009
Hammerhead (HH) ribozymes can be used for highly specific inhibition of gene expression through the degradation of target mRNA. In vitro experiments with minimal HH domains demonstrated that the efficiency of catalysis is highly dependent on concentration of magnesium ions. Optimal ion requirements for HH-catalysed RNA cleavage are far from these found
Agnieszka Fedoruk-Wyszomirska, M. Szymanski, E. Wyszko, M. Barciszewska, J. Barciszewski   +4 more
semanticscholar   +3 more sources

Hammerhead ribozyme engineering

Current Opinion in Structural Biology, 1996
Of all the catalytic RNAs the hammerhead ribozyme is the most chemically modified and structurally studied. Such studies have resulted in improvements in the nuclease resistance of ribozymes, reductions in their size, and improvements in their catalytic efficiency.
N, Usman, L, Beigelman, J A, McSwiggen
openaire   +2 more sources