Results 181 to 190 of about 16,494 (236)
Some of the next articles are maybe not open access.
Biochemical Society Transactions, 2002
The hammerhead ribozyme is a small RNA motif consisting of three helices that intersect at a conserved core. When correctly folded, the hammerhead ribozyme stimulates nearly complete cleavage of the phosphodiester chain at a defined internal site to give 2′,3′-cyclic and 5′-hydroxy termini. The cleavage rate is approx.
K F, Blount, O C, Uhlenbeck
openaire +2 more sources
The hammerhead ribozyme is a small RNA motif consisting of three helices that intersect at a conserved core. When correctly folded, the hammerhead ribozyme stimulates nearly complete cleavage of the phosphodiester chain at a defined internal site to give 2′,3′-cyclic and 5′-hydroxy termini. The cleavage rate is approx.
K F, Blount, O C, Uhlenbeck
openaire +2 more sources
Biopolymers, 1999
The hammerhead ribozyme is an intriguing RNA molecule with the ability to serve as a catalyst to cleave sequence-specifically RNA molecules in an intermolecular reaction. Preferentially Mg(2+) is required for optimal activity by inducing the catalytically competent conformation and by possibly acting as an acid-base catalyst.
F, Eckstein, B, Bramlage
openaire +2 more sources
The hammerhead ribozyme is an intriguing RNA molecule with the ability to serve as a catalyst to cleave sequence-specifically RNA molecules in an intermolecular reaction. Preferentially Mg(2+) is required for optimal activity by inducing the catalytically competent conformation and by possibly acting as an acid-base catalyst.
F, Eckstein, B, Bramlage
openaire +2 more sources
Cytotoxic Hammerhead Ribozymes
Antisense and Nucleic Acid Drug Development, 1999Small catalytic RNA molecules of the hammerhead ribozyme type were found to have cytotoxic effects unrelated to their intended activity. An expression library of ribozyme sequence variants was constructed in a recA-deficient strain of Escherichia coli such that individual library members differed in regions designed to form base pairs with human ...
R, Levitz +9 more
openaire +2 more sources
Photochemical hammerhead ribozyme activation
Bioorganic & Medicinal Chemistry Letters, 2006We report the light-activation of allosteric cis and trans acting ribozymes via decaging of a small organic molecule ligand. To achieve this effectively, we introduce an optimized N-caging group based on a nitrobenzyl core structure. This approach can potentially be employed toward a light-induced control of gene function.
Douglas D, Young, Alexander, Deiters
openaire +2 more sources
Allosteric Hammerhead Ribozyme TRAPs
Biochemistry, 2002A new mode of allosteric regulation of nucleic acid enzymes is described and shown to operate effectively with hammerhead ribozymes. In the "TRAP" design (for targeted ribozyme-attenuated probe), a 3' terminal "attenuator" anneals to conserved bases in the catalytic core to form the "off" state of the ribozyme.
Donald H, Burke +2 more
openaire +2 more sources
An Allosteric Hammerhead Ribozyme
Nature Biotechnology, 1995We have constructed an RNA molecule containing a hammerhead ribozyme that is under allosteric control. In the inactive state, the RNA enzyme is unable to cleave a suitable substrate. The formation of the active state of the ribozyme is triggered by a specific interaction with a DNA oligonucleotide effector that is complementary to a single-stranded ...
H, Porta, P M, Lizardi
openaire +2 more sources
Small, Efficient Hammerhead Ribozymes
Molecular Biotechnology, 2000The hammerhead ribozyme is able to cleave RNA in a sequence-specific manner. These ribozymes are usually designed with four basepairs in helix II, and with equal numbers of nucleotides in the 5' and 3' hybridizing arms that bind the RNA substrate on either side of the cleavage site.
M J, McCall +5 more
openaire +2 more sources
Hammerhead ribozyme design and application
Cellular and Molecular Life Sciences CMLS, 1998The emerging knowledge about RNA-based enzymes has already had great impact on our concept of evolutionary history, making the 'RNA world' more likely. It may well have an equally important impact on the diagnostic and therapeutic practices of human and veterinary medicine in the next decade. We are not quite there yet. This review addresses the design
M, Amarzguioui, H, Prydz
openaire +2 more sources
Genomisch codierte Hammerhead-Ribozyme
BIOspektrum, 2012The hammerhead ribozyme is a small RNA motif capable to cleave RNA endonucleolytically. Originally discovered in sub-viral plant pathogens, recent studies have uncovered the presence of this ribozyme in an unprecedented number of bacterial and eukaryal genomes.
Carsten Seehafer +2 more
openaire +1 more source
Hammerhead Ribozyme-Based Target Discovery
2006With the accumulation of vast amounts of data as a result of the sequencing of the human genome, it is necessary to identify human genes that are involved in various cellular, developmental, and disease-related processes and to clarify their functions and potential utility as targets in the treatment of disease.
Masayuki, Sano, Kazunari, Taira
openaire +2 more sources