Results 191 to 200 of about 16,494 (236)

Characterization of Hammerhead Ribozyme Reactions

2012
Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes.
Anne, Kalweit, Rita, Przybilski, Carsten, Seehafer, Marcos, de la Peña, Christian, Hammann   +4 more
openaire   +2 more sources

Engineering high-speed allosteric hammerhead ribozymes

bchm, 2007
Abstract Full-length hammerhead ribozymes were subjected to in vitro selection to identify variants that are allosterically regulated by theophylline in the presence of a physiologically relevant concentration of Mg2+. The population of allosteric ribozymes resulting from 15 rounds of in vitro selection yielded variants with observed ...
Kristian H, Link, Lixia, Guo, Tyler D, Ames, Laising, Yen, Richard C, Mulligan, Ronald R, Breaker   +5 more
openaire   +2 more sources

Protein Enhancement of Hammerhead Ribozyme Catalysis

Science, 1993
When the recognition sequence of a ribozyme is extended beyond a certain length, turnover is slowed and specificity is decreased. Here, it is shown that a protein can help a ribozyme overcome these general limitations on ribozyme activity. Cleavage of an RNA oligonucleotide by a hammerhead ribozyme is enhanced 10- to 20-fold upon addition of a protein ...
Z, Tsuchihashi, M, Khosla, D, Herschlag
openaire   +2 more sources

Hammerhead ribozymes for target validation

Expert Opinion on Therapeutic Targets, 2002
Expensive failures in the pharmaceutical industry might be avoided by target validation at an early stage. Often, the full consequences of inhibiting a chosen drug target do not emerge until late in the development process. One option is to use hammerhead ribozymes as highly specific ribonucleases targeted exclusively at the mRNA encoding the target ...
openaire   +3 more sources

The Hammerhead Ribozyme Structure Brought in Line

ChemBioChem, 2006
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Rita, Przybilski, Christian, Hammann
openaire   +2 more sources

The hammerhead ribozyme

Biochemical Society Transactions, 1996
J. B. Thomson, T. Tuschl, F. Eckstein
openaire   +3 more sources

Mechanistic Studies on Hammerhead Ribozymes

1996
Catalytic RNAs include group I and II introns; the RNA subunit of RNase P; hammerhead, hairpin, and hepatitis delta virus ribozymes; and ribosomal RNA (Altman 1989; Cech 1989; Michel et al. 1989; Noller et al. 1992; Symons 1992; Bratty et al. 1993; Gesteland and Atkins 1993).
P. K. R. Kumar, D. M. Zhou, K. Yoshinari, K. Taira   +3 more
openaire   +1 more source

Cooperative Interactions in the Hammerhead Ribozyme Drive pKa Shifting of G12 and Its Stacked Base C17.

Biochemistry, 2017
Erica A. Frankel, Christopher A. Strulson, C. Keating, P. Bevilacqua   +3 more
semanticscholar   +1 more source

Minimized Hammerhead Ribozymes

2003
M J, McCall, P, Hendry, T J, Lockett
openaire   +2 more sources

Structural composition of hammerhead ribozymes.

Journal of biochemistry, 1995
Eleven kinds of hammerhead ribozymes were designed and synthesized to investigate the structural composition of the complexes and to find suitable crystallization conditions, the substrate chains having been modified to prevent hydrolysis. Electrophoresis patterns indicated that the two strands except for the substrate chain form a binary complex and ...
A, Takenaka, O, Matsumoto, Y, Chen, S, Hasegawa, T, Chatake, M, Tsunoda, T, Ohta, Y, Komatsu, M, Koizumi, E, Ohtsuka   +9 more
openaire   +1 more source