Results 61 to 70 of about 16,494 (236)

Dual Promoters Improve the Rescue of Recombinant Measles Virus in Human Cells

Viruses, 2021
Reverse genetics is a technology that allows the production of a virus from its complementary DNA (cDNA). It is a powerful tool for analyzing viral genes, the development of novel vaccines, and gene delivery vectors.
Soroth Chey, Juliane Maria Palmer, Laura Doerr, Uwe Gerd Liebert   +3 more
doaj   +1 more source

Preliminary crystallographic studies on hammerhead ribozymes [PDF]

Acta Crystallographica Section A Foundations of Crystallography, 1993
Crystallization of hammerhead ribozymes was tried by the hanging drop vapor diffusion method. The crystals grown up to 0.5 x 0.05 x 0.05 mm3 in size gave diffraction patterns with about 5 A resolution using synchrotron radiation. The crystal was determined to be trigonal with space group P3(1) or P3(2) and cell parameters a = b = 49.6 A and c = 53.3 A.
O, Matsumoto, Y, Chen, S, Hasegawa, A, Takenaka, T, Ohta, M, Koizumi, E, Ohtsuka   +6 more
openaire   +2 more sources

Growth, Dissolution and Segregation of Genetically Encoded RNA Droplets by Ribozyme Catalysis

Angewandte Chemie International Edition, EarlyView.
Ribozymes enable sequence‐encoded turnover of RNA droplet material, resulting in transiently active droplets that selectively segregate based on their genotype. This establishes a direct link between genetic information and droplet behavior, marking a step toward programmable, evolvable RNA‐based compartments with life‐like properties.
Franziska Giessler, William Verstraeten, Tobias Abele, Stefan J. Maurer, Luca Monari, Kerstin Göpfrich   +5 more
wiley   +1 more source

Rational Design Strategies for Stimuli‐Responsive DNAzymes Using Modified and Artificial Nucleotides

Chemistry – A European Journal, EarlyView.
Activity control of RNA‐cleaving DNAzymes in response to external stimuli has attracted growing interest for applications in DNA‐based diagnostics, therapeutics, and dynamic DNA nanotechnology. This review summarizes rational design strategies for stimuli‐responsive DNAzymes using modified or artificial nucleotides, including split and caged DNAzymes ...
Yusuke Takezawa, Mitsuhiko Shionoya
wiley   +1 more source

The Establishment of a Tobramycin-Responsive Whole-Cell Micro-Biosensor Based on an Artificial Ribozyme Switch

Life, 2023
In this study, a tobramycin concentration-dependent whole-cell micro-biosensor (tob-HHAz) was constructed by fusing a tobramycin aptamer with a hammerhead ribozyme (HHR) from Schistosoma mansoni.
Zhenzhen Wang, Jiawen Cheng, Guimin Dai, Xiaoqi Sun, Xueli Yin, Yuanyuan Zhang   +5 more
doaj   +1 more source

Distinct 5′ UTR Requirements for Translation of the Bicistronic X/P mRNA Among Avian Orthobornaviruses

Microbiology and Immunology, EarlyView.
ABSTRACT Orthobornaviruses express X and the phosphoprotein (P) from a bicistronic X/P mRNA, and these proteins regulate polymerase activity. In mammalian orthobornaviruses, the 5′ untranslated region (5′ UTR) of the X/P mRNA controls the translational balance between X and P and thereby promotes efficient replication.
Meng‐Chi Wu, Takehiro Kanda, Ryo Komorizono, Madoka Sakai, Alexander Leacy, Leonardo Susta, Akiko Makino, Keizo Tomonaga   +7 more
wiley   +1 more source

Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus

Viruses, 2020
Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme ...
Anja Dalmann, Kerstin Wernike, Eric J. Snijder, Nadia Oreshkova, Ilona Reimann, Martin Beer   +5 more
doaj   +1 more source

A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture

RNA Biology
Self-cleaving ribozymes are versatile tools for synthetic biologists when it comes to controlling gene expression. Up to date, 12 different classes are known, and over the past decades more and more details about their structure, cleavage mechanisms and ...
Dennis Kläge, Elisabeth Müller, Jörg S. Hartig   +2 more
doaj   +1 more source

A Field‐Based Investigation of the Association Between Cotton Leafroll Dwarf Virus and Bronze Wilt‐Like Responses in Upland Cotton in Georgia

Plant Pathology, Volume 75, Issue 1, January/February 2026.
In Georgia, commercial upland cotton cultivars that expressed bronze wilt‐like responses consistently showed high copy numbers of cotton leafroll dwarf virus (CLRDV) gene, particularly in late‐planted cotton. These observations parallel historical reports of bronze wilt and suggest an association between CLRDV infection and bronze wilt‐like symptom ...
Surendra R. Edula, Sudeep Bag, Lavesta C. Hand, Peng W. Chee, John L. Snider, Robert C. Kemerait, Phillip M. Roberts   +6 more
wiley   +1 more source

Specificity of hammerhead ribozyme cleavage. [PDF]

The EMBO Journal, 1996
To be effective in gene inactivation, the hammerhead ribozyme must cleave a complementary RNA target without deleterious effects from cleaving non-target RNAs that contain mismatches and shorter stretches of complementarity. The specificity of hammerhead cleavage was evaluated using HH16, a well-characterized ribozyme designed to cleave a target of 17 ...
K J, Hertel, D, Herschlag, O C, Uhlenbeck   +2 more
openaire   +2 more sources