Synthetic circRNA therapeutics: innovations, strategies, and future horizons
MedComm, Volume 5, Issue 11, November 2024.This pie chart visually represents the comprehensive content of this review, categorized into four main sections: circRNA synthesis, circRNA optimizations, delivery platforms, and circRNA therapeutics. Each section is further divided into specific subtopics, facilitating a quick and clear understanding of the key points covered in this review. circRNA,
Jingsheng Cai +9 more
wiley +1 more source
Redesigned and chemically-modified hammerhead ribozymes with improved activity and serum stability [PDF]
, 2004 BACKGROUND: Hammerhead ribozymes are RNA-based molecules which bind and cleave other RNAs specifically. As such they have potential as laboratory reagents, diagnostics and therapeutics.
Hendry, Philip +3 more
core +2 more sources
A versatile cis-blocking and trans-activation strategy for ribozyme characterization [PDF]
, 2013 Synthetic RNA control devices that use ribozymes as gene-regulatory components have been applied to controlling cellular behaviors in response to environmental signals.
Kennedy, Andrew B. +2 more
core +1 more source
Low-magnesium, trans-cleavage activity by type III, tertiary stabilized hammerhead ribozymes with stem 1 discontinuities [PDF]
, 2005 BACKGROUND: Low concentrations of free magnesium in the intracellular environment can present critical limitations for hammerhead ribozymes, especially for those that are designed for intermolecular (trans) cleavage of a host or pathogen RNA.
Burke, Donald H, Greathouse, S Travis
core +2 more sources
Tertiary contacts distant from the active site prime a ribozyme for catalysis [PDF]
, 2006 Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis.
Martick, Monika, Scott, William G
core
Capturing Hammerhead Ribozyme Structures in Action by Modulating General Base Catalysis [PDF]
, 2008 We have obtained precatalytic (enzyme–substrate complex) and postcatalytic (enzyme–product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal.
Adams +42 more
core +4 more sources
Probing the hammerhead ribozyme structure with ribonucleases
Nucleic Acids Research, 1994 Susceptibility to RNase digestion has been used to probe the conformation of the hammerhead ribozyme structure prepared from chemically synthesised RNAs. Less than about 1.5% of the total sample was digested to obtain a profile of RNase digestion sites.
Neil J. Shirley +2 more
openaire +4 more sources
Phylogenetic footprinting of non-coding RNA: hammerhead ribozyme sequences in a satellite DNA family of Dolichopoda cave crickets (Orthoptera, Rhaphidophoridae) [PDF]
, 2010 Background The great variety in sequence, length, complexity, and abundance of satellite DNA has made it difficult to ascribe any function to this genome component.
Bachmann, Lutz +3 more
core +3 more sources
Searching genomes for ribozymes and riboswitches [PDF]
, 2007 A discussion of experimental approaches and theoretical difficulties in the identification of ribozymes with novel catalytic ...
Hammann, Christian, Westhof, Eric
core +3 more sources
Hammerhead ribozyme-based U-insertion and deletion RNA editing assays for multiplexing in HTS applications. [PDF]
RNA, 2023 Rostamighadi M +4 more
europepmc +1 more source