Results 71 to 80 of about 19,908 (281)
Complete RNA inverse folding: computational design of functional hammerhead ribozymes
, 2014 Nanotechnology and synthetic biology currently constitute one of the most innovative, interdisciplinary fields of research, poised to radically transform society in the 21st century. This paper concerns the synthetic design of ribonucleic acid molecules,
Clote, Peter +5 more
core +1 more source
Design and Preparation of a Multimeric Self-Cleaving Hammerhead Ribozyme
BioTechniques, 1997 The activity of a ribozyme can be impaired by additional sequences at the 5′ and 3′ termini of the catalytic sequence. To approach this problem, a system was designed that minimizes sequences upstream and downstream from active regions of a hammerhead ...
Juan Ruiz +3 more
doaj +1 more source
Dual Promoters Improve the Rescue of Recombinant Measles Virus in Human Cells
Viruses, 2021 Reverse genetics is a technology that allows the production of a virus from its complementary DNA (cDNA). It is a powerful tool for analyzing viral genes, the development of novel vaccines, and gene delivery vectors.
Soroth Chey +3 more
doaj +1 more source
Role of tautomerism in RNA biochemistry [PDF]
, 2015 Heterocyclic nucleic acid bases and their analogs can adopt multiple tautomeric forms due to the presence of multiple solvent-exchangeable protons.
Essigmann, John M. +2 more
core +1 more source
Parasites & Vectors, 2019 Background Giardia duodenalis causes giardiasis, with diarrhea as the primary symptom. The trophozoite proliferation of this zoonotic parasite is mainly affected by telomerase, although the mechanism of telomerase regulation has not been thoroughly ...
Jing-Tong Zheng +10 more
doaj +1 more source
Viruses, 2020 Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme ...
Anja Dalmann +5 more
doaj +1 more source
Exploring the Potential and Advancements of Circular RNA Therapeutics
Exploration, EarlyView.Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang +6 more
wiley +1 more source
Angewandte Chemie, Volume 137, Issue 8, February 17, 2025.Synthetic biomolecular condensates are appealing candidates to replicate key features and functions of intracellular membrane‐less organelles. Their dynamic phase‐separation control provides crucial insights into the regulation of fundamental cell processes.
Siyu Song +6 more
wiley +2 more sources
RNA elements and their biotechnological applications in plants
New Phytologist, EarlyView.Summary Engineering of plants for improved traits and efficient heterologous protein production can be achieved by modifying or introducing cis‐ or trans‐acting RNA elements. The function of these elements depends not only on their nucleotide sequence but also on their highly dynamic higher order structures.
Filip Lastovka +3 more
wiley +1 more source
Virology Journal, 2009 Outbreaks of Dengue impose a heavy economic burden on developing countries in terms of vector control and human morbidity. Effective vaccines against all four serotypes of Dengue are in development, but population replacement with transgenic vectors ...
Mohammed Ahmed +10 more
doaj +1 more source