Results 91 to 100 of about 2,098,712 (312)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Cycles in metabolism and heat loss [PDF]
Using calorimetric techniques, subjects' metabolism, thermoregulation, and body temperatures were monitored continuously for 24-hour days, using three types of experimental routines.
Annis, J. F., Troutman, S. J., Webb, P.
core +1 more source
FEBS Open Bio, EarlyView.Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li +8 more
wiley +1 more source
Heat Transfer Modeling of Hydrogen-Fueled Spark Ignition Engine
EnergiesCurrently, green hydrogen, generated through renewable energy sources, stands out as one of the best substitutes for fossil fuels in mitigating pollutant emissions and consequent global warming. Particularly, the utilization of hydrogen in spark ignition
Stefano Beccari, Emiliano Pipitone
doaj +1 more source
Ecology and Thermal Inactivation of Microbes in and on Interplanetary Space Vehicle Components [PDF]
Spores of Bacillus subtilis var. niger were heat treated in aqueous suspension at 90 C, and observed for morphological changes and loss of viability. The 5 logs reduction that occurred in broth at 90 min required 210 min in buffered water.
Campbell, J. E., Reyes, A. L.
core +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Buildings, 2017 Evidence of a fabric performance gap has underlined the need for measurements of in situ building performance. Steady state co-heating tests have been used since the 1980s to measure whole building heat transfer coefficients, but are often cited as ...
Samuel Stamp +2 more
doaj +1 more source
Diffusion flame extinction in slow convenctive flow under microgravity environment [PDF]
A theoretical analysis is presented to study the extinction characteristics of a diffusion flame near the leading edge of a thin fuel plate in slow, forced convective flows in a microgravity environment.
Chen, C. H.
core +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Heat loss through the window frames of buildings
Инженерно-строительный журнал, 2019 The object of investigation is window frames of buildings since they are critical zones in terms of thermal insulation. It was studied how the properties of window frame affect the change in heat flow and temperature fields. It was analyzed the heat loss
Albert Baiburin +2 more
doaj +1 more source